| Post Translational Modifications | Phosphorylated by FAM20C in the extracellular medium. Proteolytically cleaved by ADAMTS5 and ADAMTS15 in the pericellular matrix surrounding myoblasts, facilitating myoblast contact and fusion which is required for skeletal muscle development and regeneration. |
| Function | May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid. |
| Protein Name | Versican Core ProteinChondroitin Sulfate Proteoglycan Core Protein 2Chondroitin Sulfate Proteoglycan 2Glial Hyaluronate-Binding ProteinGhapLarge Fibroblast ProteoglycanPg-M |
| Database Links | Reactome: R-HSA-1971475Reactome: R-HSA-2022870Reactome: R-HSA-2022923Reactome: R-HSA-2024101Reactome: R-HSA-3000178Reactome: R-HSA-3560783Reactome: R-HSA-3560801Reactome: R-HSA-3595172Reactome: R-HSA-3595174Reactome: R-HSA-3595177Reactome: R-HSA-381426Reactome: R-HSA-4420332Reactome: R-HSA-8957275 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixCell ProjectionCiliumPhotoreceptor Outer SegmentInterphotoreceptor Matrix |
| Alternative ELISA Names | Versican Core Protein ELISA kitChondroitin Sulfate Proteoglycan Core Protein 2 ELISA kitChondroitin Sulfate Proteoglycan 2 ELISA kitGlial Hyaluronate-Binding Protein ELISA kitGhap ELISA kitLarge Fibroblast Proteoglycan ELISA kitPg-M ELISA kitVCAN ELISA kitCSPG2 ELISA kit |
| Specificity | This kit recognizes Human VCAN in samples. No significant cross-reactivity or interference between Human VCAN and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-98 | 92 |
| EDTA plasma (n=8) | 92-104 | 98 |
| Cell culture media (n=8) | 88-101 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.92 | 2.97 | 8.70 | 0.87 | 3.16 | 9.15 |
| Standard deviation | 0.05 | 0.13 | 0.39 | 0.06 | 0.17 | 0.38 |
| CV (%) | 5.43 | 4.38 | 4.48 | 6.90 | 5.38 | 4.15 |
