Human uPA/PLAU protein (Recombinant) (C-His) (STJP020049)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human uPA/PLAU Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser21-Leu431) of Human uPA/PLAU (Accession #P00749-1) fused with a C-His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human uPA/PLAU-C-His protein was developed in hek293 cells using the region Ser21-Leu431. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from 0.22 Mu m filtered solution in 1% HCOOH, 1 mM DTT (pH 3.0). Normally 8% trehalose is added as protectant before lyophilization. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | PLAU |
| Gene ID | 5328 |
| Uniprot ID | UROK_HUMAN |
| Immunogen | Recombinant Human uPA/PLAU Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser21-Leu431) of Human uPA/PLAU (Accession #P00749-1) fused with a C-His tag at the C-terminus. |
| Immunogen Region | Ser21-Leu431 |
| Immunogen Sequence | SNELHQVPSNCDCLNGGTCV SNKYFSNIHWCNCPKKFGGQ HCEIDKSKTCYEGNGHFYRG KASTDTMGRPCLPWNSATVL QQTYHAHRSDALQLGLGKHN YCRNPDNRRRPWCYVQVGLK LLVQECMVHDCADGKKPSSP PEELKFQCGQKTLRPRFKII GGEFTTIENQPWFAAIYRRH RGGSVTYVCGGSLISPCWVI SATHCFIDYPKKEDYIVYLG RSRLNSNTQGEMKFEVENL |
Additional Info
| Tissue Specificity | Expressed in the prostate gland and prostate cancers. |
| Post Translational Modifications | Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. Produced as an inactive single-chain protein (pro-uPA or sc-uPA), is processed into the active disulfide-linked two-chain form of PLAU/uPA by a proteolytic event mediated, at least, by TMPRSS4. |
| Function | Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin. |
| Protein Name | Urokinase-Type Plasminogen ActivatorU-Plasminogen ActivatorUpa Cleaved Into - Urokinase-Type Plasminogen Activator Long Chain A - Urokinase-Type Plasminogen Activator Short Chain A - Urokinase-Type Plasminogen Activator Chain B |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-75205 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Urokinase-Type Plasminogen Activator proteinU-Plasminogen Activator proteinUpa Cleaved Into - Urokinase-Type Plasminogen Activator Long Chain A - Urokinase-Type Plasminogen Activator Short Chain A - Urokinase-Type Plasminogen Activator Chain B proteinPLAU protein |
Information sourced from Uniprot.org