| Short Description : | Recombinant-Human UBE2N-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications: | ELISA/Immunogen/SDS-PAGE/WB |
| Host: | E.coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Dilution Range: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin: | Please contact us for further information. |
| Gene Symbol: | UBE2N |
| Gene ID: | 7334 |
| Uniprot ID: | UBE2N_HUMAN |
| Immunogen: | Homo sapiens (Human) |
| Immunogen Region: | Met1-Ile152 |
| Function | The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. Together with RNF135 and UB2V1, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIGI to activate the downstream signaling pathway that leads to interferon beta production. UBE2V1-UBE2N together with TRAF3IP2 E3 ubiquitin ligase mediate 'Lys-63'-linked polyubiquitination of TRAF6, a component of IL17A-mediated signaling pathway. |
| Protein Name | Ubiquitin-Conjugating Enzyme E2 NBendless-Like Ubiquitin-Conjugating EnzymeE2 Ubiquitin-Conjugating Enzyme NUbc13Ubch13Ubiquitin Carrier Protein NUbiquitin-Protein Ligase N |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-168638Reactome: R-HSA-168927Reactome: R-HSA-202424Reactome: R-HSA-2871837Reactome: R-HSA-445989Reactome: R-HSA-450302Reactome: R-HSA-450321Reactome: R-HSA-5205685Reactome: R-HSA-5607764Reactome: R-HSA-5693565Reactome: R-HSA-5693571Reactome: R-HSA-5693607Reactome: R-HSA-5696395Reactome: R-HSA-69473Reactome: R-HSA-8866654Reactome: R-HSA-9020702Reactome: R-HSA-937039Reactome: R-HSA-937041Reactome: R-HSA-9646399Reactome: R-HSA-9705671Reactome: R-HSA-975110Reactome: R-HSA-975144Reactome: R-HSA-983168 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Protein Names | Ubiquitin-Conjugating Enzyme E2 N proteinBendless-Like Ubiquitin-Conjugating Enzyme proteinE2 Ubiquitin-Conjugating Enzyme N proteinUbc13 proteinUbch13 proteinUbiquitin Carrier Protein N proteinUbiquitin-Protein Ligase N proteinUBE2N proteinBLU protein |
Information sourced from Uniprot.org
