Applications: |
CLIA |
Reactivity: |
Human |
Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: |
46.88pg/mL |
Detection Limit: |
78.13~5000pg/mL |
Short Description: |
This human TNNI3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: |
3.5h |
Detection: |
Chemiluminescence |
Gene Symbol: |
TNNI3 |
Gene ID: |
7137 |
Uniprot ID: |
TNNI3_HUMAN |
Specificity: |
This kit recognizes Human cTn-I/TNNI3 in samples. No significant cross-reactivity or interference between Human cTn-I/TNNI3 and analogues was observed. |
Sample Type: |
Serum, plasma and other biological fluids |
Post Translational Modifications | Phosphorylated at Ser-42 and Ser-44 by PRKCE.phosphorylation increases myocardium contractile dysfunction. Phosphorylated at Ser-23 and Ser-24 by PRKD1.phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). |
Function | Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. |
Protein Name | Troponin I - Cardiac MuscleCardiac Troponin I |
Database Links | Reactome: R-HSA-390522Reactome: R-HSA-5578775 |
Alternative CLIA Names | Troponin I - Cardiac Muscle CLIA kitCardiac Troponin I CLIA kitTNNI3 CLIA kitTNNC1 CLIA kit |
Specificity | This kit recognizes Human cTn-I/TNNI3 in samples. No significant cross-reactivity or interference between Human cTn-I/TNNI3 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 100-116 | 107 |
EDTA plasma(n=8) | 92-106 | 99 |
Cell culture media(n=8) | 93-107 | 98 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 254.53 | 513.41 | 1903.30 | 257.46 | 557.97 | 1898.33 |
Standard deviation | 24.89 | 60.02 | 156.64 | 30.05 | 44.30 | 184.52 |
CV (%) | 9.78 | 11.69 | 8.23 | 11.67 | 7.94 | 9.72 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance