Human TNFSF12/TWEAK protein (Recombinant) (No tag) (STJP001438)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenRecombinant Human TNFSF12/TWEAK Protein is produced by E. coli expression system. The target protein is expressed with sequence (Lys97-His249 ( N-Met) ) of human TWEAK/TNFSF12 (Accession #NP_003800.1) fused with no additional amino acid.
General Information
| Short Description | Recombinant-Human TNFSF12/TWEAK-No tag protein was developed in e.coli using the region Lys97-His249. For use in research applications. |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | 1.Measured by its binding ability in a functional ELISA. Immobilized Human TNFSF12 at 2 Mu g/mL (100 Mu L/well) can bind Human TNFRSF12A with a linear range of 0.1-2.3 ng/mL.|2.Measured in a cell proliferation assay using human umbilical vein endothe |
| Determination Method | <0.1 EU/Mu g |
Target Information
| Gene Symbol | TNFSF12 |
| Gene ID | 8742 |
| Uniprot ID | TNF12_HUMAN |
| Immunogen | Recombinant Human TNFSF12/TWEAK Protein is produced by E. coli expression system. The target protein is expressed with sequence (Lys97-His249 ( N-Met) ) of human TWEAK/TNFSF12 (Accession #NP_003800.1) fused with no additional amino acid. |
| Immunogen Region | Lys97-His249 |
| Immunogen Sequence | KGRKTRARRAIAAHYEVHPR PGQDGAQAGVDGTVSGWEEA RINSSSPLRYNRQIGEFIVT RAGLYYLYCQVHFDEGKAVY LKLDLLVDGVLALRCLEEFS ATAASSLGPQLRLCQVSGLL ALRPGSSLRIRTLPWAHLKA APFLTYFGLFQVH |
Additional Info
| Post Translational Modifications | The soluble form derives from the membrane form by proteolytic processing. |
| Function | Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion. |
| Protein Name | Tumor Necrosis Factor Ligand Superfamily Member 12Apo3 LigandTnf-Related Weak Inducer Of ApoptosisTweak Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 12 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 12 - Secreted Form |
| Database Links | Reactome: R-HSA-5668541Reactome: R-HSA-5676594 |
| Cellular Localisation | Cell MembraneSingle-Pass Type Ii Membrane ProteinTumor Necrosis Factor Ligand Superfamily Member 12Secreted Form: SecretedIsoform Twe-Pril: Cell MembraneSingle-Pass Membrane Protein |
| Alternative Protein Names | Tumor Necrosis Factor Ligand Superfamily Member 12 proteinApo3 Ligand proteinTnf-Related Weak Inducer Of Apoptosis proteinTweak Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 12 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 12 - Secreted Form proteinTNFSF12 proteinAPO3L proteinDR3LG proteinUNQ181 proteinPRO207 protein |
Information sourced from Uniprot.org