Human TNFRSF7/CD27 protein (Recombinant) (C-hFc & His) (STJP002752)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human TNFRSF7/CD27 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Thr21-Ile192) of human CD27/TNFRSF7 (Accession #NP_001233.1) fused with a Fc, 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human TNFRSF7/CD27-C-hFc & His protein was developed in hek293 cells using the region Thr21-Ile192. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. Immobilized Human CD27 at 2 Mu g/mL (100 Mu L/well) can bind Human CD70 with a linear range of 0.781-0.885 ng/mL. |
| Determination Method | <0.1 EU/Mu g |
Target Information
| Gene Symbol | CD27 |
| Gene ID | 939 |
| Uniprot ID | CD27_HUMAN |
| Immunogen | Recombinant Human TNFRSF7/CD27 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Thr21-Ile192) of human CD27/TNFRSF7 (Accession #NP_001233.1) fused with a Fc, 6×His tag at the C-terminus. |
| Immunogen Region | Thr21-Ile192 |
| Immunogen Sequence | TPAPKSCPERHYWAQGKLCC QMCEPGTFLVKDCDQHRKAA QCDPCIPGVSFSPDHHTRPH CESCRHCNSGLLVRNCTITA NAECACRNGWQCRDKECTEC DPLPNPSLTARSSQALSPHP QPTHLPYVSEMLEARTAGHM QTLADFRQLPARTLSTHWPP QRSLCSSDFIRI |
Additional Info
| Post Translational Modifications | Phosphorylated. N-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. |
| Function | Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells. Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory. In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis. Negatively regulates the function of Th2 lymphocytes in the adipose tissue. |
| Protein Name | Cd27 AntigenCd27l ReceptorT-Cell Activation Antigen Cd27T14Tumor Necrosis Factor Receptor Superfamily Member 7Cd Antigen Cd27 |
| Database Links | Reactome: R-HSA-5669034 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Cd27 Antigen proteinCd27l Receptor proteinT-Cell Activation Antigen Cd27 proteinT14 proteinTumor Necrosis Factor Receptor Superfamily Member 7 proteinCd Antigen Cd27 proteinCD27 proteinTNFRSF7 protein |
Information sourced from Uniprot.org