Human TNFRSF1B/TNF-R2/CD120b protein (Recombinant) (C-His) (STJP001021)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human TNFRSF1B/TNF-R2/CD120b Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu 23-Asp 257) of human TNFR2 (Accession #NP_001057.1) fused with a 6ΓHis tag at the C-terminus.
General Information
| Short Description | Recombinant-Human TNFRSF1B/TNF-R2/CD120b-C-His protein was developed in hek293 cells using the region Leu 23-Asp 257. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20Β°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its ability to inhibit TNF-Alpha mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 1-3 Mu g/mL in the presence of 0.25 ng/mL |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | TNFRSF1B |
| Gene ID | 7133 |
| Uniprot ID | TNR1B_HUMAN |
| Immunogen | Recombinant Human TNFRSF1B/TNF-R2/CD120b Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu 23-Asp 257) of human TNFR2 (Accession #NP_001057.1) fused with a 6ΓHis tag at the C-terminus. |
| Immunogen Region | Leu 23-Asp 257 |
| Immunogen Sequence | LPAQVAFTPYAPEPGSTCRL REYYDQTAQMCCSKCSPGQH AKVFCTKTSDTVCDSCEDST YTQLWNWVPECLSCGSRCSS DQVETQACTREQNRICTCRP GWYCALSKQEGCRLCAPLRK CRPGFGVARPGTETSDVVCK PCAPGTFSNTTSSTDICRPH QICNVVAIPGNASMDAVCTS TSPTRSMAPGAVHLPQPVST RSQHTQPTPEPSTAPSTSFL LPMGPSPPAEGSTGD |
Additional Info
| Function | Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. |
| Protein Name | Tumor Necrosis Factor Receptor Superfamily Member 1bTumor Necrosis Factor Receptor 2Tnf-R2Tumor Necrosis Factor Receptor Type IiTnf-RiiTnfr-IiP75P80 Tnf-Alpha ReceptorCd Antigen Cd120bEtanercept Cleaved Into - Tumor Necrosis Factor Receptor Superfamily Member 1b - Membrane Form - Tumor Necrosis Factor-Binding Protein 2Tbp-2Tbpii |
| Database Links | Reactome: R-HSA-5668541Reactome: R-HSA-5669034Reactome: R-HSA-6783783Reactome: R-HSA-6785807Reactome: R-HSA-6798695 |
| Cellular Localisation | Isoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinIsoform 2: SecretedTumor Necrosis Factor-Binding Protein 2: Secreted |
| Alternative Protein Names | Tumor Necrosis Factor Receptor Superfamily Member 1b proteinTumor Necrosis Factor Receptor 2 proteinTnf-R2 proteinTumor Necrosis Factor Receptor Type Ii proteinTnf-Rii proteinTnfr-Ii proteinP75 proteinP80 Tnf-Alpha Receptor proteinCd Antigen Cd120b proteinEtanercept Cleaved Into - Tumor Necrosis Factor Receptor Superfamily Member 1b - Membrane Form - Tumor Necrosis Factor-Binding Protein 2 proteinTbp-2 proteinTbpii proteinTNFRSF1B proteinTNFBR proteinTNFR2 protein |
Information sourced from Uniprot.org