Human TNFRSF1A (Tumor necrosis factor receptor superfamily member 1A) Sandwich ELISA Kit (STJE0002062)

Name: Human TNFRSF1A (Tumor necrosis factor receptor superfamily member 1A) Sandwich ELISA Kit (STJE0002062)
SKU: STJE0002062
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0002062

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General Information

Short Description	This TNFRSF1A Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	4.69pg/mL
Detection Limit	7.81~500pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Target Information

Gene Symbol	TNFRSF1A
Gene ID	7132
Uniprot ID	TNR1A_HUMAN
Specificity	This kit recognizes Human TNFRSF1A in samples. No significant cross-reactivity or interference between Human TNFRSF1A and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Additional Info

Function	Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
Protein Name	Tumor Necrosis Factor Receptor Superfamily Member 1a Tumor Necrosis Factor Receptor 1 Tnf-R1 Tumor Necrosis Factor Receptor Type I Tnf-Ri Tnfr-I P55 P60 Cd Antigen Cd120a Cleaved Into - Tumor Necrosis Factor Receptor Superfamily Member 1a - Membrane Form - Tumor Necrosis Factor-Binding Protein 1 Tbpi
Database Links	Reactome: R-HSA-5357786 Reactome: R-HSA-5357905 Reactome: R-HSA-5357956 Reactome: R-HSA-5626978 Reactome: R-HSA-5669034 Reactome: R-HSA-6783783 Reactome: R-HSA-75893
Cellular Localisation	Cell Membrane Single-Pass Type I Membrane Protein Golgi Apparatus Membrane Secreted A Secreted Form Is Produced Through Proteolytic Processing Isoform 4: Secreted Lacks A Golgi-Retention Motif Is Not Membrane Bound And Therefore Is Secreted
Alternative ELISA Names	Tumor Necrosis Factor Receptor Superfamily Member 1a ELISA kit Tumor Necrosis Factor Receptor 1 ELISA kit Tnf-R1 ELISA kit Tumor Necrosis Factor Receptor Type I ELISA kit Tnf-Ri ELISA kit Tnfr-I ELISA kit P55 ELISA kit P60 ELISA kit Cd Antigen Cd120a Cleaved Into - Tumor Necrosis Factor Receptor Superfamily Member 1a - Membrane Form - Tumor Necrosis Factor-Binding Protein 1 ELISA kit Tbpi ELISA kit TNFRSF1A ELISA kit TNFAR ELISA kit TNFR1 ELISA kit
Specificity	This kit recognizes Human TNFRSF1A in samples. No significant cross-reactivity or interference between Human TNFRSF1A and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	88-103	95
EDTA plasma (n=8)	94-108	101
Cell culture media (n=8)	92-103	97

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	23.21	74.91	187.90	20.91	67.85	199.93
Standard deviation	1.27	3.03	7.27	1.40	4.05	10.96
CV (%)	5.47	4.04	3.87	6.70	5.97	5.48

Citations

View product citations for antibody STJE0002062 on CiteAb