• Human TLR2 (Toll-like receptor 2) CLIA Kit (STJC0000471)

Human TLR2 (Toll-like receptor 2) CLIA Kit (STJC0000471)

SKU:
STJC0000471

Shipping:
Free Shipping
Current Stock:
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This human TLR2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: TLR2
Gene ID: 7097
Uniprot ID: TLR2_HUMAN
Specificity: This kit recognizes Human TLR2 in samples. No significant cross-reactivity or interference between Human TLR2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
Post Translational Modifications Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2. Ubiquitinated at Lys-754 by PPP1R11, leading to its degradation. Deubiquitinated by USP2.
Function Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also activate immune cells and promote apoptosis in response to the lipid moiety of lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. Stimulation of monocytes in vitro with M.tuberculosis PstS1 induces p38 MAPK and ERK1/2 activation primarily via this receptor, but also partially via TLR4. MAPK activation in response to bacterial peptidoglycan also occurs via this receptor. Acts as a receptor for M.tuberculosis lipoproteins LprA, LprG, LpqH and PstS1, some lipoproteins are dependent on other coreceptors (TLR1, CD14 and/or CD36).the lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen. M.tuberculosis HSP70 (dnaK) but not HSP65 (groEL-2) acts via this protein to stimulate NF-kappa-B expression. Recognizes M.tuberculosis major T-antigen EsxA (ESAT-6) which inhibits downstream MYD88-dependent signaling (shown in mouse). Forms activation clusters composed of several receptors depending on the ligand, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Forms the cluster TLR2:TLR6:CD14:CD36 in response to diacylated lipopeptides and TLR2:TLR1:CD14 in response to triacylated lipopeptides. Required for normal uptake of M.tuberculosis, a process that is inhibited by M.tuberculosis LppM.
Protein Name Toll-Like Receptor 2
Toll/Interleukin-1 Receptor-Like Protein 4
Cd Antigen Cd282
Database Links Reactome: R-HSA-1236974
Reactome: R-HSA-1461957
Reactome: R-HSA-166058
Reactome: R-HSA-168179
Reactome: R-HSA-168188
Reactome: R-HSA-5602498
Reactome: R-HSA-5603041
Reactome: R-HSA-5686938
Reactome: R-HSA-6798695
Reactome: R-HSA-9637628
Reactome: R-HSA-9705671
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Cytoplasmic Vesicle
Phagosome Membrane
Membrane Raft
Does Not Reside In Lipid Rafts Before Stimulation But Accumulates Increasingly In The Raft Upon The Presence Of The Microbial Ligand
In Response To Diacylated Lipoproteins
Tlr2:Tlr6 Heterodimers Are Recruited In Lipid Rafts
This Recruitment Determines The Intracellular Targeting To The Golgi Apparatus
Triacylated Lipoproteins Induce The Same Mechanism For Tlr2:Tlr1 Heterodimers
Alternative CLIA Names Toll-Like Receptor 2 CLIA kit
Toll/Interleukin-1 Receptor-Like Protein 4 CLIA kit
Cd Antigen Cd282 CLIA kit
TLR2 CLIA kit
TIL4 CLIA kit
Specificity This kit recognizes Human TLR2 in samples. No significant cross-reactivity or interference between Human TLR2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 100-113 106
EDTA plasma(n=8) 98-116 106
Cell culture media(n=8) 94-107 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 198.61 560.41 1772.46 204.13 592.31 1738.18
Standard deviation 21.47 46.40 141.62 22.86 60.12 114.20
CV (%) 10.81 8.28 7.99 11.20 10.15 6.57
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance