| Host: | HEK293 |
| Note: | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human Tissue kallikrein-protein was developed from hek293. For use in research applications. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Dilution Range: | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: | The activity was tested by the ability to cleave a fluorogenic substrate PFR-AMC.The specific activity was measured to be 1.2 nmole/ug/min NA |
| Gene Symbol: | KLK1 |
| Gene ID: | 3816 |
| Uniprot ID: | KLK1_HUMAN |
| Immunogen Region: | PRO19-SER262 |
| Immunogen: | Optimized DNA sequence encoding Human Tissue kallikrein-KLK1 (PRO19-SER262) preprotein including a C-terminal His tag was expressed in HEK293 cells. NA |
| Tissue Specificity | Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84. |
| Post Translational Modifications | The O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located. |
| Function | Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. (Microbial infection) Cleaves Neisseria meningitidis NHBA in saliva.Neisseria is an obligate commensal of the nasopharyngeal mucosa. |
| Protein Name | Kallikrein-1Kidney/Pancreas/Salivary Gland KallikreinTissue Kallikrein |
| Database Links | Reactome: R-HSA-381426 |
| Alternative Protein Names | Kallikrein-1 proteinKidney/Pancreas/Salivary Gland Kallikrein proteinTissue Kallikrein proteinKLK1 protein |
Information sourced from Uniprot.org
