| Short Description : | Recombinant-Human THSD1-C-His protein was developed in hek293 cells using the region Glu 25-Ile 361. For use in research applications. |
| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: | Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. When 5×104 cells/well are added to rhTHSD1 coated plates (30 µg/mL, 100 µL/well) , approximately 13-17% will adhere after 60 minu |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Gene Symbol: | THSD1 |
| Gene ID: | 55901 |
| Uniprot ID: | THSD1_HUMAN |
| Immunogen: | Recombinant Human THSD1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu 25-Ile 361 ) of human THSD1 (Accession #NP_954872.1) fused with a 6×His tag at the C-terminus. |
| Immunogen Region: | Glu 25-Ile 361 |
| Immunogen Sequence: | EYLLLREPGHVALSNDTVYV DFQYFDGANGTLRNVSVLLL EANTNQTVTTKYLLTNQSQG TLKFECFYFKEAGDYWFTMT PEATDNSTPFPWWEKSAFLK VEWPVFHVDLNRSAKAAEGT FQVGLFTSQPLCPFPVDKPN IVVDVIFTNSLPEARRNSRQ PLEIRTSKRTELAQGQWVEF GCAPLGPEAYVTVVLKLLGR DSVITSTGPIDLAQKFGYKL VMVPELTCESGVEVTVLPP |
| Function | Is a positive regulator of nascent focal adhesion assembly, involved in the modulation of endothelial cell attachment to the extracellular matrix. |
| Protein Name | Thrombospondin Type-1 Domain-Containing Protein 1Transmembrane Molecule With Thrombospondin Module |
| Database Links | Reactome: R-HSA-5083635Reactome: R-HSA-5173214 |
| Cellular Localisation | Isoform 1: Endosome MembraneSingle-Pass Type I Membrane ProteinCell JunctionFocal AdhesionLocalizes To Nascent Focal AdhesionsIsoform 2: MembraneIsoform 3: Secreted |
| Alternative Protein Names | Thrombospondin Type-1 Domain-Containing Protein 1 proteinTransmembrane Molecule With Thrombospondin Module proteinTHSD1 proteinTMTSP proteinUNQ3010 proteinPRO9769 protein |
Information sourced from Uniprot.org
