| Short Description : | This TGFA Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids. |
| Applications: | ELISA |
| Reactivity: | Human |
| Sensitivity: | 18.75pg/mL |
| Detection Limit: | 31.25~2000pg/mL |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | TGFA |
| Gene ID: | 7039 |
| Uniprot ID: | TGFA_HUMAN |
| Specificity: | This kit recognizes Human TGF-Alpha in samples. No significant cross-reactivity or interference between Human TGF-Alpha and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. |
| Protein Name | Protransforming Growth Factor Alpha Cleaved Into - Transforming Growth Factor AlphaTgf-AlphaEgf-Like TgfEtgfTgf Type 1 |
| Database Links | Reactome: R-HSA-1257604Reactome: R-HSA-177929Reactome: R-HSA-179812Reactome: R-HSA-180292Reactome: R-HSA-180336Reactome: R-HSA-182971Reactome: R-HSA-204005Reactome: R-HSA-212718Reactome: R-HSA-2219530Reactome: R-HSA-5638303Reactome: R-HSA-5673001Reactome: R-HSA-5694530Reactome: R-HSA-6811558Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8866910Reactome: R-HSA-9009391Reactome: R-HSA-9018519Reactome: R-HSA-9634638 |
| Cellular Localisation | Transforming Growth Factor Alpha: SecretedExtracellular SpaceProtransforming Growth Factor Alpha: Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative ELISA Names | Protransforming Growth Factor Alpha Cleaved Into - Transforming Growth Factor Alpha ELISA kitTgf-Alpha ELISA kitEgf-Like Tgf ELISA kitEtgf ELISA kitTgf Type 1 ELISA kitTGFA ELISA kit |
| Specificity | This kit recognizes Human TGF-α in samples. No significant cross-reactivity or interference between Human TGF-α and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 86-99 | 92 |
| EDTA plasma (n=8) | 90-103 | 95 |
| Cell culture media (n=8) | 92-108 | 99 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 100.31 | 184.40 | 675.41 | 91.03 | 184.52 | 658.62 |
| Standard deviation | 6.40 | 7.38 | 24.79 | 5.26 | 10.55 | 20.15 |
| CV (%) | 6.38 | 4.00 | 3.67 | 5.78 | 5.72 | 3.06 |
