| Host: | E.coli |
| Applications: | SDS-PAGE |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human TAF10-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Formulation: | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% Glycerol, 1mM DTT |
| Concentration: | 0.25 mg/mL |
| Storage Instruction: | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TAF10 |
| Gene ID: | 6881 |
| Uniprot ID: | TAF10_HUMAN |
| Immunogen Region: | 84-218aa |
| Accession Number: | NP_006275 |
| Immunogen: | Human |
| Immunogen Sequence: | MGSSHHHHHH SSGLVPRGSH MGSGAAPPEG AISNGVYVLP SAANGDVKPV VSSTPLVDFL MQLEDYTPTI PDAVTGYYLN RAGFEASDPR IIRLISLAAQ KFISDIANDA LQHCKMKGTA SGSSRSKSKD RKYTLTMEDL TPALSEYGIN VKKPHYFT |
| Post Translational Modifications | Monomethylated at Lys-189 by SETD7, leading to increased affinity for RNA polymerase II. Lysine deamination at Lys-189 to form allysine is mediated by LOXL2. Allysine formation by LOXL2 results in release of TAF10 from promoters, leading to inhibition of TFIID-dependent transcription. |
| Function | The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. TAF10 is also component of the PCAF histone acetylase complex, the TATA-binding protein-free TAF complex (TFTC) and the STAGA transcription coactivator-HAT complex. May regulate cyclin E expression. |
| Protein Name | Transcription Initiation Factor Tfiid Subunit 10Staf28Transcription Initiation Factor Tfiid 30 Kda SubunitTaf(Ii30Tafii-30Tafii30 |
| Database Links | Reactome: R-HSA-167161Reactome: R-HSA-167162Reactome: R-HSA-167172Reactome: R-HSA-3214847Reactome: R-HSA-5689880Reactome: R-HSA-674695Reactome: R-HSA-6804756Reactome: R-HSA-73776Reactome: R-HSA-73779Reactome: R-HSA-75953Reactome: R-HSA-76042 |
| Cellular Localisation | Nucleus |
| Alternative Protein Names | Transcription Initiation Factor Tfiid Subunit 10 proteinStaf28 proteinTranscription Initiation Factor Tfiid 30 Kda Subunit proteinTaf(Ii30 proteinTafii-30 proteinTafii30 proteinTAF10 proteinTAF2A proteinTAF2H proteinTAFII30 protein |
Information sourced from Uniprot.org