| Post Translational Modifications | O-glycosylated. |
| Function | Actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity. |
| Protein Name | Synaptopodin |
| Cellular Localisation | CytoplasmCytoskeletonCell JunctionTight JunctionPerikaryonCell ProjectionDendritic SpinePostsynaptic DensitySynapseCytosolLocalized At The Tight Junction Of CellsIn BrainLocalized To The Postsynaptic Densities And In The PerikaryaAssociated With Dendritic Spines Of A Subset Of Synapses |
| Alternative ELISA Names | Synaptopodin ELISA kitSYNPO ELISA kitKIAA1029 ELISA kit |
| Specificity | This kit recognizes Human SYNPO in samples. No significant cross-reactivity or interference between Human SYNPO and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-110 | 100 |
| EDTA plasma (n=8) | 86-98 | 93 |
| Cell culture media (n=8) | 90-106 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.46 | 1.51 | 4.56 | 0.47 | 1.58 | 4.59 |
| Standard deviation | 0.02 | 0.06 | 0.18 | 0.02 | 0.07 | 0.17 |
| CV (%) | 4.35 | 3.97 | 3.95 | 4.26 | 4.43 | 3.70 |
