| Protein Name | SparcBasement-Membrane Protein 40Bm-40OsteonectinOnSecreted Protein Acidic And Rich In Cysteine |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1251985Reactome: R-HSA-3000178Reactome: R-HSA-3000497 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixBasement MembraneIn Or Around The Basement Membrane |
| Alternative ELISA Names | Sparc ELISA kitBasement-Membrane Protein 40 ELISA kitBm-40 ELISA kitOsteonectin ELISA kitOn ELISA kitSecreted Protein Acidic And Rich In Cysteine ELISA kitSPARC ELISA kitON ELISA kit |
| Specificity | This kit recognizes Human ON in samples. No significant cross-reactivity or interference between Human ON and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-101 | 96 |
| EDTA plasma (n=8) | 88-102 | 93 |
| Cell culture media (n=8) | 89-104 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.08 | 1.91 | 8.12 | 1.14 | 1.95 | 7.33 |
| Standard deviation | 0.07 | 0.11 | 0.38 | 0.06 | 0.10 | 0.37 |
| CV (%) | 6.48 | 5.76 | 4.68 | 5.26 | 5.13 | 5.05 |
