Human SNC Alpha (Alpha-Synuclein) Antibody Pair (STJAP00450)
General Information
| Short Description | This SNC Alpha antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | SNCA |
| Gene ID | 6622 |
| Uniprot ID | SYUA_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human SNC Alpha protien.Detection Immunogen: Recombinant Human SNC Alpha protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress. Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers. Ubiquitinated. The predominant conjugate is the diubiquitinated form. Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. |
| Function | Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity. |
| Protein Name | Alpha-SynucleinNon-A Beta Component Of Ad AmyloidNon-A4 Component Of Amyloid PrecursorNacp |
| Database Links | Reactome: R-HSA-977225Reactome: R-HSA-9833482 |
| Cellular Localisation | CytoplasmMembraneNucleusSynapseSecretedCell ProjectionAxonMembrane-Bound In Dopaminergic NeuronsExpressed And Colocalized With Septin4 In Dopaminergic Axon TerminalsEspecially At The Varicosities |
| Alternative Antibody Pair Names | Alpha-Synuclein antibody pairNon-A Beta Component Of Ad Amyloid antibody pairNon-A4 Component Of Amyloid Precursor antibody pairNacp antibody pairSNCA antibody pairNACP antibody pairPARK1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human SNC Alpha (Alpha-Synuclein) Antibody Pair (STJAP00450)
This antibody pair detects human Alpha-Synuclein (SNC Alpha, UniProt P37840) and has confirmed reactivity with human samples only. Both the capture and detection antibodies were raised against recombinant human SNC Alpha protein.
This antibody pair is validated for ELISA. Recommended sample types include serum, plasma, and other biological fluids.
The capture antibody is supplied unconjugated at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5. The detection antibody is biotin-conjugated at 0.5 mg/mL in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
Both the capture and detection antibodies were purified by antigen affinity chromatography. Each was raised against recombinant human SNC Alpha protein as the immunogen.
Both components should be stored at -20°C for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain antibody integrity.
No. This product is for research use only and is not intended for diagnostic, therapeutic, or clinical procedures.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.