• Biotinylated Human SIRP alpha V4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
  • The purity of Biotinylated Human SIRP alpha V4 is greater than 95% as determined by SEC-HPLC.
  • Immobilized Human CD47, hFc Tag at 1 Mu g/ml (100 Mu l/Well) on the plate. Dose response curve for Biotinylated Human SIRP alpha V4, His Tag with the EC50 of 0. 47 Mu g/ml determined by ELISA.

Human SIRP alpha V4 protein (Recombinant) (C-His-Avi) {Biotin} (STJP001760)

SKU:
STJP001760

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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human SIRP alpha V4-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Immunogen Region: Glu31-Arg370 (E33G, L44S, T50S, T52I, R54H)
Immunogen: DNA sequence encoding Human SIRP alpha V4 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Post Translational Modifications N-glycosylated. Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11.
Function Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Protein Name Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1
Shp Substrate 1
Shps-1
Brain Ig-Like Molecule With Tyrosine-Based Activation Motifs
Bit
Cd172 Antigen-Like Family Member A
Inhibitory Receptor Shps-1
Macrophage Fusion Receptor
Myd-1 Antigen
Signal-Regulatory Protein Alpha-1
Sirp-Alpha-1
Signal-Regulatory Protein Alpha-2
Sirp-Alpha-2
Signal-Regulatory Protein Alpha-3
Sirp-Alpha-3
P84
Cd Antigen Cd172a
Database Links Reactome: R-HSA-202733
Reactome: R-HSA-391160
Reactome: R-HSA-6798695
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Protein Names Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1 protein
Shp Substrate 1 protein
Shps-1 protein
Brain Ig-Like Molecule With Tyrosine-Based Activation Motifs protein
Bit protein
Cd172 Antigen-Like Family Member A protein
Inhibitory Receptor Shps-1 protein
Macrophage Fusion Receptor protein
Myd-1 Antigen protein
Signal-Regulatory Protein Alpha-1 protein
Sirp-Alpha-1 protein
Signal-Regulatory Protein Alpha-2 protein
Sirp-Alpha-2 protein
Signal-Regulatory Protein Alpha-3 protein
Sirp-Alpha-3 protein
P84 protein
Cd Antigen Cd172a protein
SIRPA protein
BIT protein
MFR protein
MYD1 protein
PTPNS1 protein
SHPS1 protein
SIRP protein

Information sourced from Uniprot.org

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