| Host: |
HEK293 cells |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human SIRP-alpha/CD172a-C-His protein was developed from hek293 cells and has a target region of C-His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. Contact us for customized product form or formulation. |
| Immunoreactivity: |
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human SIRPA at 5 Mu g/mL (100 Mu L/well) can bind Recombinant Human CD47 with a linear range of 0.1-0.4 Mu g/mL. |
| Gene Symbol: |
SIRPA |
| Gene ID: |
140885 |
| Uniprot ID: |
SHPS1_HUMAN |
| Immunogen: |
Recombinant Human SIRP-alpha/CD172a Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Glu31-Arg370) of human SIRP alpha/CD172a (Accession #NP_542970) fused with a 6×His tag at the C-terminus. |
| Tissue Specificity | Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. |
| Post Translational Modifications | N-glycosylated. Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. |
| Function | Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Plays a role in antiviral immunity and limits new world arenavirus infection by decreasing virus internalization. Receptor for THBS1. Interaction with THBS1 stimulates phosphorylation of SIRPA. In response to THBS1, involved in ROS signaling in non-phagocytic cells, stimulating NADPH oxidase-derived ROS production. |
| Protein Name | Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1Shp Substrate 1Shps-1Brain Ig-Like Molecule With Tyrosine-Based Activation MotifsBitCd172 Antigen-Like Family Member AInhibitory Receptor Shps-1Macrophage Fusion ReceptorMyd-1 AntigenSignal-Regulatory Protein Alpha-1Sirp-Alpha-1Signal-Regulatory Protein Alpha-2Sirp-Alpha-2Signal-Regulatory Protein Alpha-3Sirp-Alpha-3P84Cd Antigen Cd172a |
| Database Links | Reactome: R-HSA-202733Reactome: R-HSA-391160Reactome: R-HSA-6798695 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1 proteinShp Substrate 1 proteinShps-1 proteinBrain Ig-Like Molecule With Tyrosine-Based Activation Motifs proteinBit proteinCd172 Antigen-Like Family Member A proteinInhibitory Receptor Shps-1 proteinMacrophage Fusion Receptor proteinMyd-1 Antigen proteinSignal-Regulatory Protein Alpha-1 proteinSirp-Alpha-1 proteinSignal-Regulatory Protein Alpha-2 proteinSirp-Alpha-2 proteinSignal-Regulatory Protein Alpha-3 proteinSirp-Alpha-3 proteinP84 proteinCd Antigen Cd172a proteinSIRPA proteinBIT proteinMFR proteinMYD1 proteinPTPNS1 proteinSHPS1 proteinSIRP protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
