| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This human SIGLEC8 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
SIGLEC8 |
| Gene ID: |
27181 |
| Uniprot ID: |
SIGL8_HUMAN |
| Specificity: |
This kit recognizes Human SIGLEC8 in samples. No significant cross-reactivity or interference between Human SIGLEC8 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed specifically on red blood cells namely basophil, mast cells and eosinophils. |
| Function | Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose. Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes. |
| Protein Name | Sialic Acid-Binding Ig-Like Lectin 8Siglec-8Sialoadhesin Family Member 2Saf-2 |
| Database Links | Reactome: R-HSA-198933 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative CLIA Names | Sialic Acid-Binding Ig-Like Lectin 8 CLIA kitSiglec-8 CLIA kitSialoadhesin Family Member 2 CLIA kitSaf-2 CLIA kitSIGLEC8 CLIA kitSAF2 CLIA kit |
| Specificity | This kit recognizes Human SIGLEC8 in samples. No significant cross-reactivity or interference between Human SIGLEC8 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 96-106 | 101 |
| EDTA plasma(n=8) | 90-101 | 96 |
| Cell culture media(n=8) | 94-111 | 102 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 185.11 | 414.94 | 1622.13 | 190.03 | 452.56 | 1515.54 |
| Standard deviation | 16.40 | 46.31 | 98.95 | 21.80 | 43.67 | 104.42 |
| CV (%) | 8.86 | 11.16 | 6.10 | 11.47 | 9.65 | 6.89 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
