Name: Human SIGLEC8 (Sialic acid-binding Ig-like lectin 8) CLIA Kit (STJC0000610)
SKU: STJC0000610
Availability: InStock

Applications:	CLIA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	37.5pg/mL
Detection Limit:	62.5~4000pg/mL
Short Description :	This human SIGLEC8 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	SIGLEC8
Gene ID:	27181
Uniprot ID:	SIGL8_HUMAN
Specificity:	This kit recognizes Human SIGLEC8 in samples. No significant cross-reactivity or interference between Human SIGLEC8 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Expressed specifically on blood cells namely basophil, mast cells and eosinophils.
Function	Putative adhesion molecule that mediates sialic-acid dependent binding to blood cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose. Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes.
Protein Name	Sialic Acid-Binding Ig-Like Lectin 8 Siglec-8 Sialoadhesin Family Member 2 Saf-2
Database Links	Reactome: R-HSA-198933
Cellular Localisation	Membrane Single-Pass Type I Membrane Protein
Alternative CLIA Names	Sialic Acid-Binding Ig-Like Lectin 8 CLIA kit Siglec-8 CLIA kit Sialoadhesin Family Member 2 CLIA kit Saf-2 CLIA kit SIGLEC8 CLIA kit SAF2 CLIA kit
Specificity	This kit recognizes Human SIGLEC8 in samples. No significant cross-reactivity or interference between Human SIGLEC8 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	185.11	414.94	1622.13	190.03	452.56	1515.54
Standard deviation	16.40	46.31	98.95	21.80	43.67	104.42
CV (%)	8.86	11.16	6.10	11.47	9.65	6.89

Model	Name	Reactivity	Application
STJC0000540	Human FABP7 (Fatty acid-binding protein, brain) CLIA Kit (STJC0000540)	Human	CLIA
STJC0000635	Human FABP3 (Fatty acid-binding protein, heart) CLIA Kit (STJC0000635)	Human	CLIA
STJC0000902	Human IgE (Immunoglobulin E) CLIA Kit (STJC0000902)	Human	CLIA
STJC0000259	Human IGFBP1 (Insulin-like growth factor-binding protein 1) CLIA Kit (STJC0000259)	Human	CLIA
STJC0000263	Human IGFBP7 (Insulin-like growth factor-binding protein 7) CLIA Kit (STJC0000263)	Human	CLIA

Human SIGLEC8 (Sialic acid-binding Ig-like lectin 8) CLIA Kit (STJC0000610)