Human SIGLEC8/SAF-2 protein (Recombinant-Active) (C-His) (STJP012294)
SPECIFICATIONS
HostMammalian Cells
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Active-Human SIGLEC8/SAF-2-C-His protein was developed from mammalian cells and has a target region of C-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | Mammalian Cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | <0.1 EU/Mu g of the protein by the LAL method. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. When biotinylated Sialyl Lewis X was Immobilised at 0.05 Mu g/mL (100 Mu L/well) , it binds recombinant human SIGLEC8/SAF-2. The concentration of human SIGLEC8/SAF-2 that produces 50% of the opti |
Target Information
| Gene Symbol | SIGLEC8 |
| Gene ID | 27181 |
| Uniprot ID | SIGL8_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met17-Ala363 |
Additional Info
| Function | Putative adhesion molecule that mediates sialic-acid dependent binding to blood cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose. Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes. |
| Protein Name | Sialic Acid-Binding Ig-Like Lectin 8Siglec-8Sialoadhesin Family Member 2Saf-2 |
| Database Links | Reactome: R-HSA-198933 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Sialic Acid-Binding Ig-Like Lectin 8 proteinSiglec-8 proteinSialoadhesin Family Member 2 proteinSaf-2 proteinSIGLEC8 proteinSAF2 protein |
Information sourced from Uniprot.org