| Short Description : | Recombinant-Human Siglec-8-C-His&Avi protein was developed in hek293 cells using the region Met17-Ala363. For use in research applications. |
| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Biotin |
| Formulation: | Lyophilised from 0.22 Mu m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 1 EU/Mu g of the protein by LAL method. |
| Gene Symbol: | SIGLEC8 |
| Gene ID: | 27181 |
| Uniprot ID: | SIGL8_HUMAN |
| Immunogen: | Biotinylated Recombinant Human Siglec-8 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met17-Ala363) of Human Siglec-8 (Accession #Q9NYZ4) fused with a C-His&Avi tag at the C-terminus. |
| Immunogen Region: | Met17-Ala363 |
| Immunogen Sequence: | MEGDRQYGDGYLLQVQELVT VQEGLCVHVPCSFSYPQDGW TDSDPVHGYWFRAGDRPYQD APVATNNPDREVQAETQGRF QLLGDIWSNDCSLSIRDARK RDKGSYFFRLERGSMKWSYK SQLNYKTKQLSVFVTALTHR PDILILGTLESGHSRNLTCS VPWACKQGTPPMISWIGASV SSPGPTTARSSVLTLTPKPQ DHGTSLTCQVTLPGTGVTTT STVRLDVSYPPWNLTMTVF |
| Tissue Specificity | Expressed specifically on blood cells namely basophil, mast cells and eosinophils. |
| Function | Putative adhesion molecule that mediates sialic-acid dependent binding to blood cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose. Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes. |
| Protein Name | Sialic Acid-Binding Ig-Like Lectin 8Siglec-8Sialoadhesin Family Member 2Saf-2 |
| Database Links | Reactome: R-HSA-198933 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Protein Names | Sialic Acid-Binding Ig-Like Lectin 8 proteinSiglec-8 proteinSialoadhesin Family Member 2 proteinSaf-2 proteinSIGLEC8 proteinSAF2 protein |
Information sourced from Uniprot.org
