Human SHH protein (Recombinant) (Tag free) (STJP014641)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human SHH-Tag free protein was developed from e.coli and has a target region of Tag free. For use in research applications. |
| Applications | ELISA/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a 0.22 Mu m filtered solution in 20mM PB, 100mM NaCl, 1mM DTT, pH 7.5. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8ยฐC for one week. Store at-20 to-80ยฐC for twelve months from the date of receipt. |
| Endotoxin | < 0.01 EU/ยตg as determined by LAL test. |
Target Information
| Gene Symbol | SHH |
| Gene ID | 6469 |
| Uniprot ID | SHH_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Cys24-Gly197 |
Additional Info
| Function | Sonic hedgehog protein: The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN. Both activities occur in the endoplasmic reticulum. Once cleaved, ShhC is degraded in the endoplasmic reticulum. Sonic hedgehog protein N-product: The dually lipidated sonic hedgehog protein N-product (ShhNp) is a morphogen which is essential for a variety of patterning events during development. Induces ventral cell fate in the neural tube and somites. Involved in the patterning of the anterior-posterior axis of the developing limb bud. Essential for axon guidance. Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO. |
| Protein Name | Sonic Hedgehog ProteinShhHhg-1Shh Unprocessed N-Terminal Signaling And C-Terminal Autoprocessing DomainsShhnc Cleaved Into - Sonic Hedgehog Protein N-ProductShhnShh N-Terminal Processed Signaling DomainsShhnp |
| Database Links | Reactome: R-HSA-373080Reactome: R-HSA-5358346Reactome: R-HSA-5362768Reactome: R-HSA-5362798Reactome: R-HSA-5632681Reactome: R-HSA-5632684Reactome: R-HSA-5635838Reactome: R-HSA-5658034Reactome: R-HSA-9758920Reactome: R-HSA-9796292Reactome: R-HSA-9925561 |
| Cellular Localisation | Sonic Hedgehog Protein: Endoplasmic Reticulum MembraneGolgi Apparatus MembraneSecretedCo-Localizes With Hhat In The Er And Golgi MembraneSonic Hedgehog Protein N-Product: Cell MembraneLipid-AnchorThe Dual-Lipidated Sonic Hedgehog Protein N-Product (Shhnp) Is Firmly Tethered To The Cell Membrane Where It Forms MultimersFurther Solubilization And Release From The Cell Surface Seem To Be Achieved Through Different MechanismsIncluding The Interaction With Disp1 And Scube2Movement By Lipoprotein ParticlesTransport By Cellular Extensions Called Cytonemes Or By The Proteolytic Removal Of Both Terminal Lipidated Peptides |
| Alternative Protein Names | Sonic Hedgehog Protein proteinShh proteinHhg-1 proteinShh Unprocessed N-Terminal Signaling And C-Terminal Autoprocessing Domains proteinShhnc Cleaved Into - Sonic Hedgehog Protein N-Product proteinShhn proteinShh N-Terminal Processed Signaling Domains proteinShhnp proteinSHH protein |
Information sourced from Uniprot.org