Human SFTPA1 (Pulmonary surfactant-associated protein A1) CLIA Kit for Quantitative Detection

Name: Human SFTPA1 (Pulmonary surfactant-associated protein A1) CLIA Kit for Quantitative Detection
SKU: STJC0000577
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJC0000577

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General Information

Short Description	This human SFTPA1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications	CLIA
Reactivity	Human
Sensitivity	7.5pg/mL
Detection Limit	12.5~800pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Chemiluminescence

Target Information

Gene Symbol	SFTPA1
Gene ID	653509
Uniprot ID	SFTA1_HUMAN
Specificity	This kit recognizes Human SP-A1 in samples. No significant cross-reactivity or interference between Human SP-A1 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Additional Info

Post Translational Modifications	N-acetylated.
Function	In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages. (Microbial infection) Recognition of M.tuberculosis by dendritic cells may occur partially via this molecule. Can recognize, bind, and opsonize pathogens to enhance their elimination by alveolar macrophages. (Microbial infection) Binds M.pneumoniae CARDS toxin, serves as one receptor for this pathogen. When SFTPA1 is down-regulated by siRNA, less toxin binds to human cells and less vacuolization (a symptom of M.pneumoniae infection) is seen.
Protein Name	Pulmonary Surfactant-Associated Protein A1 Psp-A Pspa Sp-A Sp-A1 35 Kda Pulmonary Surfactant-Associated Protein Alveolar Proteinosis Protein Collectin-4
Database Links	Reactome: R-HSA-166016 Reactome: R-HSA-168179 Reactome: R-HSA-391160 Reactome: R-HSA-5683826 Reactome: R-HSA-5686938 Reactome: R-HSA-5688849 Reactome: R-HSA-5688890
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix Surface Film
Alternative CLIA Names	Pulmonary Surfactant-Associated Protein A1 CLIA kit Psp-A CLIA kit Pspa CLIA kit Sp-A CLIA kit Sp-A1 CLIA kit 35 Kda Pulmonary Surfactant-Associated Protein CLIA kit Alveolar Proteinosis Protein CLIA kit Collectin-4 CLIA kit SFTPA1 CLIA kit COLEC4 CLIA kit PSAP CLIA kit SFTP1 CLIA kit SFTPA CLIA kit SFTPA1B CLIA kit
Specificity	This kit recognizes Human SP-A1 in samples. No significant cross-reactivity or interference between Human SP-A1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-102	93
EDTA plasma(n=8)	88-100	93
Cell culture media(n=8)	96-112	104

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	39.22	85.66	274.30	40.34	93.21	286.68
Standard deviation	4.12	7.08	19.80	3.39	7.70	30.47
CV (%)	10.50	8.27	7.22	8.40	8.26	10.63

Citations

View product citations for antibody STJC0000577 on CiteAb