Human SERPINF2 (Alpha-2-antiplasmin) Sandwich ELISA Kit (STJE0002629)

Name: Human SERPINF2 (Alpha-2-antiplasmin) Sandwich ELISA Kit (STJE0002629)
SKU: STJE0002629
Availability: InStock

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SPECIFICATIONS

STJE0002629

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Short Description	This SERPINF2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	7.5ng/mL
Detection Limit	12.5~800ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	SERPINF2
Gene ID	5345
Uniprot ID	A2AP_HUMAN
Specificity	This kit recognizes Human Alpha 2-AP in samples. No significant cross-reactivity or interference between Human Alpha 2-AP and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Post Translational Modifications	Proteolytically cleaved at Pro-39 by both the prolyl endopeptidase FAP form and antiplasmin-cleaving enzyme FAP soluble form to generate mature alpha-2-antiplasmin.
Function	Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin.
Protein Name	Alpha-2-Antiplasmin Alpha-2-Ap Alpha-2-Plasmin Inhibitor Alpha-2-Pi Serpin F2
Database Links	Reactome: R-HSA-114608 Reactome: R-HSA-75205
Cellular Localisation	Secreted
Alternative ELISA Names	Alpha-2-Antiplasmin ELISA kit Alpha-2-Ap ELISA kit Alpha-2-Plasmin Inhibitor ELISA kit Alpha-2-Pi ELISA kit Serpin F2 ELISA kit SERPINF2 ELISA kit AAP ELISA kit PLI ELISA kit
Specificity	This kit recognizes Human α2-AP in samples. No significant cross-reactivity or interference between Human α2-AP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	40.88	118.96	393.16	39.63	122.85	396.21
Standard deviation	2.07	5.92	16.00	2.22	6.15	13.87
CV (%)	5.06	4.98	4.07	5.60	5.01	3.50