| Host: | Mammalian Cells |
| Applications: | ELISA/WB |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Active-Human SERPINF1/PEDF/Serpin F1-C-His protein was developed from mammalian cells and has a target region of C-His. For use in research applications. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol. |
| Dilution Range: | Reconstitute in sterile water for a stock solution. |
| Storage Instruction: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin: | <0.1 EU/Mu g of the protein by the LAL method. |
| Immunoreactivity: | Measured by its ability to enhance the adhesion of human osteosarcoma cells (Saos‑2) to bovine Collagen I coated plate. The ED50 for this effect is 0.15-0.75 Mu g/mL. |
| Gene Symbol: | SERPINF1 |
| Gene ID: | 5176 |
| Uniprot ID: | PEDF_HUMAN |
| Immunogen Region: | Gln20-Pro418 |
| Immunogen: | Homo sapiens (Human) |
| Post Translational Modifications | The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity. N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. |
| Function | Neurotrophic protein.induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. |
| Protein Name | Pigment Epithelium-Derived FactorPedfCell Proliferation-Inducing Gene 35 ProteinEpc-1Serpin F1 |
| Database Links | |
| Cellular Localisation | SecretedMelanosomeEnriched In Stage I Melanosomes |
| Alternative Protein Names | Pigment Epithelium-Derived Factor proteinPedf proteinCell Proliferation-Inducing Gene 35 Protein proteinEpc-1 proteinSerpin F1 proteinSERPINF1 proteinPEDF proteinPIG35 protein |
Information sourced from Uniprot.org