• Biotinylated Human Semaphorin 4D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
  • The purity of Biotinylated Human Semaphorin 4D is greater than 95% as determined by SEC-HPLC.
  • Immobilized Anti-Semaphorin 4D Antibody, hFc Tag at 0. 5 Mu g/ml (100 Mu l/Well) on the plate. Dose response curve for Biotinylated Human Semaphorin 4D, His Tag with the EC50 of 44. 8ng/ml determined by ELISA.

Human Semaphorin 4D/SEMA4D/CD100 protein (Recombinant) (C-His-Avi) {Biotin} (STJP001561)

SKU:
STJP001561

£618.50 - £1,909.50
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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human Semaphorin 4D/SEMA4D/CD100-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: SEMA4D
Gene ID: 10507
Uniprot ID: SEM4D_HUMAN
Immunogen Region: Met22-Arg734
Immunogen: DNA sequence encoding Human Semaphorin 4D/SEMA4D/CD100 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Function Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner. Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells. Plays a role in the immune system.induces B-cells to aggregate and improves their viability (in vitro). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway.
Protein Name Semaphorin-4d
A8
Bb18
Gr3
Cd Antigen Cd100
Database Links Reactome: R-HSA-416550
Reactome: R-HSA-416572
Reactome: R-HSA-416700
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Protein Names Semaphorin-4d protein
A8 protein
Bb18 protein
Gr3 protein
Cd Antigen Cd100 protein
SEMA4D protein
C9orf164 protein
CD100 protein
SEMAJ protein

Information sourced from Uniprot.org

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