Human SCNN1g (Amiloride Sensitive Sodium Channel Subunit Gamma) Sandwich ELISA Kit Ready-To-Use Kit (STJE0018430)
SPECIFICATIONS
General Information
| Short Description | This SCNN1g Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 0.113ng/mL |
| Detection Limit | 0.312-20ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 3 hrs |
Target Information
| Gene Symbol | SCNN1G |
| Gene ID | 6340 |
| Uniprot ID | SCNNG_HUMAN |
| Immunogen Region | Ready-To-Use |
| Sample Type | tissue homogenates or other biological fluids. |
Additional Info
| Tissue Specificity | Expressed in kidney (at protein level). |
| Post Translational Modifications | Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation. Ubiquitinated. Can be ubiquitinated at multiple sites and undergo monoubiquitination and polyubiquitination. Ubiquitination by NEDD4 or NEDD4L inhibits the ENaC channel through endocytosis, intracellular retention and degradation of its individual subunits. ENaC is activated through the proteolytic maturation of its subunits. Furin cleaves the SCNN1G subunit first, followed by cleavage by prostasin (PRSS8), which results in a stepwise increase in the open probability of the channel due to the release of an inhibitory tract. BPIFA1, which is recruited by the SCNN1B subunit, prevents the proteolytic activation of ENaC. N-glycosylated. N-linked glycans are processed to complex type during ENaC complex assembly and transport to the plasma membrane. |
| Function | This is one of the three pore-forming subunits of the heterotrimeric epithelial sodium channel (ENaC), a critical regulator of sodium balance and fluid homeostasis. ENaC operates in epithelial tissues, where it mediates the electrodiffusion of sodium ions from extracellular fluid through the apical membrane of cells, with water following osmotically. It plays a key role in maintaining sodium homeostasis through electrogenic sodium reabsorption in the kidneys. Additionally, ENaC is essential for airway surface liquid homeostasis, which is crucial for proper mucus clearance. |
| Protein Name | Epithelial Sodium Channel Subunit GammaEnac Subunit GammaEnacgEpithelial Na(+ Channel Subunit GammaGamma-EnacAmiloride-Sensitive Sodium Channel Subunit GammaGamma-NachNonvoltage-Gated Sodium Channel 1 Subunit GammaScnegSodium Channel Epithelial 1 Subunit Gamma |
| Database Links | Reactome: R-HSA-2672351Reactome: R-HSA-9730628 |
| Cellular Localisation | Apical Cell MembraneMulti-Pass Membrane Protein |
| Alternative ELISA Names | Epithelial Sodium Channel Subunit Gamma ELISA kitEnac Subunit Gamma ELISA kitEnacg ELISA kitEpithelial Na(+ Channel Subunit Gamma ELISA kitGamma-Enac ELISA kitAmiloride-Sensitive Sodium Channel Subunit Gamma ELISA kitGamma-Nach ELISA kitNonvoltage-Gated Sodium Channel 1 Subunit Gamma ELISA kitScneg ELISA kitSodium Channel Epithelial 1 Subunit Gamma ELISA kitSCNN1G ELISA kit |
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Information sourced from Uniprot.org