Human RPN1 (Ribophorin I) Sandwich ELISA Kit Ready-To-Use Kit (STJE0018329)
SPECIFICATIONS
General Information
| Short Description | This RPN1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 0.63ng/mL |
| Detection Limit | 1.56-100ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 3 hrs |
Target Information
| Gene Symbol | RPN1 |
| Gene ID | 6184 |
| Uniprot ID | RPN1_HUMAN |
| Immunogen Region | Ready-To-Use |
| Sample Type | tissue homogenates or other biological fluids. |
Additional Info
| Tissue Specificity | Expressed in all tissues tested. |
| Post Translational Modifications | Ubiquitinated by the ECS(ASB11) complex. Ubiquitinated by RNF128, leading to degradation in a proteasome/lysosome-dependent manner. Ufmylated by UFL1 in response to endoplasmic reticulum stress, promoting reticulophagy of endoplasmic reticulum sheets. |
| Function | Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. |
| Protein Name | Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Subunit 1Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase 67 Kda SubunitRibophorin IRpn-IRibophorin-1 |
| Database Links | Reactome: R-HSA-1799339Reactome: R-HSA-446203Reactome: R-HSA-9694548 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type I Membrane ProteinMelanosomeIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative ELISA Names | Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Subunit 1 ELISA kitDolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase 67 Kda Subunit ELISA kitRibophorin I ELISA kitRpn-I ELISA kitRibophorin-1 ELISA kitRPN1 ELISA kit |
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Information sourced from Uniprot.org