Human RPL5 protein (Recombinant) (His-Tag) (STJP018877)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human RPL5-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.25 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% Glycerol, 1mM DTT |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RPL5 |
| Gene ID | 6125 |
| Uniprot ID | RL5_HUMAN |
| Accession Number | NP_000960 |
| Immunogen | Human |
| Immunogen Region | 1-297aa |
| Immunogen Sequence | MGSSHHHHHH SSGLVPRGSH MGSMGFVKVV KNKAYFKRYQ VKFRRRREGK TDYYARKRLV IQDKNKYNTP KYRMIVRVTN RDIICQIAYA RIEGDMIVCA AYAHELPKYG VKVGLTNYAA AYCTGLLLAR RLLNRFGMDK IYEGQVEVTG DEYNVESIDG QPGAFTCYLD AGLARTTTGN KVFGALKGAV DGGLSIPHST KRFPGYDSES KEFNAEVHRK HIMGQNVA |
Additional Info
| Function | Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs. It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53. |
| Protein Name | Large Ribosomal Subunit Protein Ul1860s Ribosomal Protein L5 |
| Database Links | Reactome: R-HSA-156827Reactome: R-HSA-156902Reactome: R-HSA-1799339Reactome: R-HSA-192823Reactome: R-HSA-2408557Reactome: R-HSA-6791226Reactome: R-HSA-72689Reactome: R-HSA-72706Reactome: R-HSA-72764Reactome: R-HSA-9010553Reactome: R-HSA-9633012Reactome: R-HSA-975956Reactome: R-HSA-975957 |
| Cellular Localisation | CytoplasmNucleusNucleolusAlthough Rp5 Is Functional Within The CytoplasmThe Assembly Of Ribosomal Subunits Occurs In The NucleusRpl5 Nuclear Import Is Mediated By Ipo5/Ranbp5Ipo7/Ranbp7Kpnb1/Importin-Beta Or Tpno1/Trn |
| Alternative Protein Names | Large Ribosomal Subunit Protein Ul18 protein60s Ribosomal Protein L5 proteinRPL5 proteinMSTP030 protein |
Information sourced from Uniprot.org