| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.058ng/mL |
| Detection Limit: | 0.156-10ng/mL |
| Short Description : | This RPL13A Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates or other biological fluids. |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | RPL13A |
| Gene ID: | 23521 |
| Uniprot ID: | RL13A_HUMAN |
| Sample Type: | tissue homogenates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Phosphorylation at Ser-77 upon interferon-gamma treatment in monocytes involves a DAPK1-DAPK3 kinase cascade and is causing release from the ribosome, association with the GAIT complex and subsequent involvement in transcript-selective translation inhibition. Citrullinated by PADI4. |
| Function | Associated with ribosomes but is not required for canonical ribosome function and has extra-ribosomal functions. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the ribosome and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. In the GAIT complex interacts with m7G cap-bound eIF4G at or near the eIF3-binding site and blocks the recruitment of the 43S ribosomal complex. Involved in methylation of rRNA. |
| Protein Name | Large Ribosomal Subunit Protein Ul1323 Kda Highly Basic Protein60s Ribosomal Protein L13a |
| Database Links | Reactome: R-HSA-156827Reactome: R-HSA-156902Reactome: R-HSA-1799339Reactome: R-HSA-192823Reactome: R-HSA-2408557Reactome: R-HSA-6791226Reactome: R-HSA-72689Reactome: R-HSA-72706Reactome: R-HSA-72764Reactome: R-HSA-9010553Reactome: R-HSA-9633012Reactome: R-HSA-975956Reactome: R-HSA-975957 |
| Cellular Localisation | Cytoplasm |
| Alternative ELISA Names | Large Ribosomal Subunit Protein Ul13 ELISA kit23 Kda Highly Basic Protein ELISA kit60s Ribosomal Protein L13a ELISA kitRPL13A ELISA kit |
| output |
Information sourced from Uniprot.org
