Human RNF43 protein (Recombinant) (C-hFC) (STJP020349)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human RNF43 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly24-Tyr197) of human RNF43 (Accession #NP_001292473.1) fused with a hFc tag at the C-terminus.
General Information
| Short Description | Recombinant-Human RNF43-C-hFC protein was developed in hek293 cells using the region Gly24-Tyr197. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | RNF43 |
| Gene ID | 54894 |
| Uniprot ID | RNF43_HUMAN |
| Immunogen | Recombinant Human RNF43 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly24-Tyr197) of human RNF43 (Accession #NP_001292473.1) fused with a hFc tag at the C-terminus. |
| Immunogen Region | Gly24-Tyr197 |
| Immunogen Sequence | GFGRTGLVLAAAVESERSAE QKAIIRVIPLKMDPTGKLNL TLEGVFAGVAEITPAEGKLM QSHPLYLCNASDDDNLEPGF ISIVKLESPRRAPRPCLSLA SKARMAGERGASAVLFDITE DRAAAEQLQQPLGLTWPVVL IWGNDAEKLMEFVYKNQKAH VRIELKEPPAWPDY |
Additional Info
| Tissue Specificity | Expressed in fetal kidney, fetal lung, in colon cancer tissues, hepatocellular carcinomas and lung adenocarcinomas. Overexpressed in colorectal cancer cell lines. |
| Post Translational Modifications | Autoubiquitinated. |
| Function | E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Along with RSPO2 and ZNRF3, constitutes a master switch that governs limb specification. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf43Ring Finger Protein 43Ring-Type E3 Ubiquitin Transferase Rnf43 |
| Database Links | Reactome: R-HSA-4641263Reactome: R-HSA-5340588 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinEndoplasmic Reticulum MembraneNucleus EnvelopeAccording To A ReportMay Be Secreted |
| Alternative Protein Names | E3 Ubiquitin-Protein Ligase Rnf43 proteinRing Finger Protein 43 proteinRing-Type E3 Ubiquitin Transferase Rnf43 proteinRNF43 protein |
Information sourced from Uniprot.org