Human RNA polymerase III subunit RPC6/POLR3F protein (Recombinant) (His-Tag) (STJP018004)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human Rpolymerase III subunit RPC6/POLR3F-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 30% Glycerol, 1mM DTT |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | POLR3F |
| Gene ID | 10621 |
| Uniprot ID | RPC6_HUMAN |
| Accession Number | NP_006457 |
| Immunogen | Human |
| Immunogen Region | 1-316aa |
| Immunogen Sequence | MGSSHHHHHH SSGLVPRGSH MGSMAEVKVK VQPPDADPVE IENRIIELCH QFPHGITDQV IQNEMPHIEA QQRAVAINRL LSMGQLDLLR SNTGLLYRIK DSQNAGKMKG SDNQEKLVYQ IIEDAGNKGI WSRDIRYKSN LPLTEINKIL KNLESKKLIK AVKSVAASKK KVYMLYNLQP DRSVTGGAWY SDQDFESEFV EVLNQQCFKF LQSKAETARE SKQNPMIQ |
Additional Info
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. Part of POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer that coordinates the dynamics of Pol III stalk and clamp modules during the transition from apo to elongation state. Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses, including varicella zoster virus. Acts as a nuclear and cytosolic DNA sensor detecting AT-rich DNA, involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway. Preferentially binds double-stranded DNA (dsDNA). |
| Protein Name | Dna-Directed Rna Polymerase Iii Subunit Rpc6Rna Polymerase Iii Subunit C6Dna-Directed Rna Polymerase Iii Subunit FRna Polymerase Iii 39 Kda SubunitRpc39 |
| Database Links | Reactome: R-HSA-1834949Reactome: R-HSA-73780Reactome: R-HSA-73980Reactome: R-HSA-749476Reactome: R-HSA-76061Reactome: R-HSA-76066Reactome: R-HSA-76071 |
| Cellular Localisation | Nucleus |
| Alternative Protein Names | Dna-Directed Rna Polymerase Iii Subunit Rpc6 proteinRna Polymerase Iii Subunit C6 proteinDna-Directed Rna Polymerase Iii Subunit F proteinRna Polymerase Iii 39 Kda Subunit proteinRpc39 proteinPOLR3F protein |
Information sourced from Uniprot.org