| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.118ng/mL |
| Detection Limit: | 0.312-20ng/mL |
| Short Description: | This RHO Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates or other biological fluids.. |
| Storage Instruction: | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 3 hrs |
| Gene Symbol: | RHO |
| Gene ID: | 6010 |
| Uniprot ID: | OPSD_HUMAN |
| Immunogen Region: | Ready-To-Use |
| Sample Type: | tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Rod shaped photoreceptor cells which mediate vision in dim light. |
| Post Translational Modifications | Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region. After activation by light, phosphorylated by GRK1 (in vitro). Contains one covalently linked retinal chromophore. Upon light absorption, the covalently bound 11-cis-retinal is converted to all-trans-retinal. After hydrolysis of the Schiff base and release of the covalently bound all-trans-retinal, active rhodopsin is regenerated by binding of a fresh molecule of 11-cis-retinal. |
| Function | Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling. |
| Protein Name | Rhodopsin Opsin-2 |
| Database Links | Reactome: R-HSA-2453902 Reactome: R-HSA-2485179 Reactome: R-HSA-2514859 Reactome: R-HSA-418594 Reactome: R-HSA-419771 Reactome: R-HSA-5620916 |
| Cellular Localisation | Membrane Multi-Pass Membrane Protein Cell Projection Cilium Photoreceptor Outer Segment Synthesized In The Inner Segment (Is) Of Rod Photoreceptor Cells Before Vectorial Transport To Disk Membranes In The Rod Outer Segment (Os) Photosensory Cilia |
| Alternative ELISA Names | Rhodopsin ELISA kit Opsin-2 ELISA kit RHO ELISA kit OPN2 ELISA kit |
| output |
Information sourced from Uniprot.org
