Human RHO (Rhodopsin) Sandwich ELISA Kit Ready-To-Use Kit (STJE0018272)
SPECIFICATIONS
General Information
| Short Description | This RHO Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 0.118ng/mL |
| Detection Limit | 0.312-20ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 3 hrs |
Target Information
| Gene Symbol | RHO |
| Gene ID | 6010 |
| Uniprot ID | OPSD_HUMAN |
| Immunogen Region | Ready-To-Use |
| Sample Type | tissue homogenates, cell lysates or other biological fluids. |
Additional Info
| Tissue Specificity | Rod shaped photoreceptor cells which mediate vision in dim light. |
| Post Translational Modifications | Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region. After activation by light, phosphorylated by GRK1 (in vitro). Contains one covalently linked retinal chromophore. Upon light absorption, the covalently bound 11-cis-retinal is converted to all-trans-retinal. After hydrolysis of the Schiff base and release of the covalently bound all-trans-retinal, active rhodopsin is regenerated by binding of a fresh molecule of 11-cis-retinal. |
| Function | Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling. |
| Protein Name | RhodopsinOpsin-2 |
| Database Links | Reactome: R-HSA-2453902Reactome: R-HSA-2485179Reactome: R-HSA-2514859Reactome: R-HSA-418594Reactome: R-HSA-419771Reactome: R-HSA-5620916 |
| Cellular Localisation | MembraneMulti-Pass Membrane ProteinCell ProjectionCiliumPhotoreceptor Outer SegmentSynthesized In The Inner Segment (Is) Of Rod Photoreceptor Cells Before Vectorial Transport To Disk Membranes In The Rod Outer Segment (Os) Photosensory Cilia |
| Alternative ELISA Names | Rhodopsin ELISA kitOpsin-2 ELISA kitRHO ELISA kitOPN2 ELISA kit |
| output |
Information sourced from Uniprot.org