This RALBP1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates or other biological fluids..
Applications
ELISA
Reactivity
Human
Sensitivity
0.058ng/mL
Detection Limit
0.156-10ng/mL
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Storage Instruction
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
tissue homogenates, cell lysates or other biological fluids.
Additional Info
Tissue Specificity
Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes.
Post Translational Modifications
Tyrosine-phosphorylated upon stimulation of cells with EGF. May undergo proteolytic cleavage to give peptides which reassemble to form a transporter complex.
Function
Multifunctional protein that functions as a downstream effector of RALA and RALB. As a GTPase-activating protein/GAP can inactivate CDC42 and RAC1 by stimulating their GTPase activity. As part of the Ral signaling pathway, may also regulate ligand-dependent EGF and insulin receptors-mediated endocytosis. During mitosis, may act as a scaffold protein in the phosphorylation of EPSIN/EPN1 by the mitotic kinase cyclin B-CDK1, preventing endocytosis during that phase of the cell cycle. During mitosis, also controls mitochondrial fission as an effector of RALA. Recruited to mitochondrion by RALA, acts as a scaffold to foster the mitotic kinase cyclin B-CDK1-mediated phosphorylation and activation of DNM1L. Could also function as a primary ATP-dependent active transporter for glutathione conjugates of electrophiles. May also actively catalyze the efflux of a wide range of substrates including xenobiotics like doxorubicin (DOX) contributing to cell multidrug resistance.
Protein Name
Rala-Binding Protein 1Ralbp176 Kda Ral-Interacting ProteinDinitrophenyl S-Glutathione AtpaseDnp-Sg AtpaseRal-Interacting Protein 1
Cell MembranePeripheral Membrane ProteinCytoplasmCytosolCytoskeletonSpindle PoleNucleusMitochondrionCytosolic Protein That Transiently Associates With The Mitotic Spindle Poles In Early ProphaseAnd Dissociates From Them After Completion Of MitosisTargeted To The Plasma Membrane Through Its Interaction With RalbDirected By Fgf SignalingDocking On The Membrane Is Required To Transduce The Ral SignalRecruited By Rala To The Mitochondrion During Mitosis Where It Regulates Mitochondrial FissionNuclear Localization Is Cell Cycle Dependent While Membrane Localization Is Seen In Adherent CellsThe Region Involved In Membrane Association Could Form Transmembrane Domains And Expose A Part Of The Protein Extracellularly (Probable)
