Human PSMD10 (Proteasome 26S Subunit, Non ATPase 10) Sandwich ELISA Kit (STJE0011714)
SPECIFICATIONS
General Information
| Short Description | This PSMD10 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 0.059ng/mL |
| Detection Limit | 0.156-10ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 4.5 hrs |
Target Information
| Gene Symbol | PSMD10 |
| Gene ID | 5716 |
| Uniprot ID | PSD10_HUMAN |
| Sample Type | tissue homogenates or other biological fluids. |
Additional Info
| Tissue Specificity | Tends to be up-regulated in cancer cells with RAS mutations, including lung cancers and adenocarconimas (at protein level). |
| Post Translational Modifications | |
| Function | Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pathway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis. Acts as an proto-oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export. |
| Protein Name | 26s Proteasome Non-Atpase Regulatory Subunit 1026s Proteasome Regulatory Subunit P28GankyrinP28(Gank |
| Database Links | Reactome: R-HSA-9907900 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative ELISA Names | 26s Proteasome Non-Atpase Regulatory Subunit 10 ELISA kit26s Proteasome Regulatory Subunit P28 ELISA kitGankyrin ELISA kitP28(Gank ELISA kitPSMD10 ELISA kit |
| output |
Information sourced from Uniprot.org