| Host: | HEK293 cells |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Recombinant-Human Proliferating cell nuclear antigen/PCNA-N-HA protein was developed from hek293 cells and has a target region of N-HA. For use in research applications. |
| Formulation: | Lyophilized from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PCNA |
| Gene ID: | 5111 |
| Uniprot ID: | PCNA_HUMAN |
| Immunogen Region: | Phe2-Ser261 |
| Immunogen: | Recombinant Human Proliferating cell nuclear antigen/PCNA Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Phe2-Ser261) of human Proliferating cell nuclear antigen/PCNA (Accession #NP_002583.1) fus |
| Function | Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. |
| Protein Name | Proliferating Cell Nuclear AntigenPcnaCyclin |
| Database Links | Reactome: R-HSA-110312Reactome: R-HSA-110314Reactome: R-HSA-110320Reactome: R-HSA-1362277Reactome: R-HSA-174411Reactome: R-HSA-174414Reactome: R-HSA-174417Reactome: R-HSA-174437Reactome: R-HSA-4615885Reactome: R-HSA-5358565Reactome: R-HSA-5358606Reactome: R-HSA-5651801Reactome: R-HSA-5655862Reactome: R-HSA-5656121Reactome: R-HSA-5656169Reactome: R-HSA-5685942Reactome: R-HSA-5696397Reactome: R-HSA-5696400Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6804114Reactome: R-HSA-69091Reactome: R-HSA-69166Reactome: R-HSA-69183Reactome: R-HSA-69205Reactome: R-HSA-8866654 |
| Cellular Localisation | NucleusColocalizes With CrebbpEp300 And Pold1 To Sites Of Dna DamageForms Nuclear Foci Representing Sites Of Ongoing Dna Replication And Vary In Morphology And Number During S PhaseCo-Localizes With Smarca5/Snf2h And Baz1b/Wstf At Replication Foci During S PhaseTogether With Apex2Is Redistributed In Discrete Nuclear Foci In Presence Of Oxidative Dna Damaging Agents |
| Alternative Protein Names | Proliferating Cell Nuclear Antigen proteinPcna proteinCyclin proteinPCNA protein |
Information sourced from Uniprot.org
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