Human Profilin 4/PFN4 protein (Recombinant) (His-Tag) (STJP016650)

SPECIFICATIONS
HostE.coli
ImmunogenHuman
STJP016650
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General Information

Short DescriptionRecombinant-Human Profilin 4/PFN4-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
ApplicationsSDS-PAGE
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Concentration0.5 mg/mL
FormulationLiquid in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% Glycerol
Storage InstructionFor short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolPFN4
Gene ID375189
Uniprot IDPROF4_HUMAN
Accession NumberNP_955378
ImmunogenHuman
Immunogen Region1-129aa
Immunogen Sequence MSHLQSLLLD TLLGTKHVDS AALIKIQERS LCVASPGFNV TPSDVRTLVN GFAKNPLQAR REGLYFKGKD YRCVRADEYS LYAKNENTGV VVVKTHLYLL VATYTEGMYP SICVEATESL GDYLRKKGS

Additional Info

Tissue Specificity Expressed in testis, in germ cells in seminiferous tubules (at protein level). Prominently expressed in pachytene/diplotene stage spematocytes.
Function Involved in male fertility. Required for manchette development and acrosome biogenesis during spermiogenesis. Binds in vitro to phospholipids, including phosphatidylinositol 3-phosphate (PtdIns(3)P), phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), phosphatidylinositol 4-phosphate (PtdIns(4)P) and phosphatidic acid (PA). Contrary to other profilin family members, does not bind to actin in vitro.
Protein Name Profilin-4
Profilin Iv
Database Links
Cellular Localisation Cytoplasm
In Round Spermatids
Mainly Observed In The Acroplaxome
During The Progression Of Spermiogenesis
Relocalizes To The Developing Manchette Of Spermatids Step 8 (S8)
Coinciding With The Initiation Of Manchette Disassembly In Spermatids S14
Seen In The Cytoplasm Subjacent To The Disassembling Manchette
Alternative Protein Names Profilin-4 protein
Profilin Iv protein
PFN4 protein

Information sourced from Uniprot.org

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