Human PRKAb1 (Protein Kinase, AMP Activated Beta 1) Sandwich ELISA Kit Ready-To-Use Kit (STJE0018046)

SPECIFICATIONS
STJE0018046
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General Information

Short DescriptionThis PRKAb1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates or other biological fluids..
ApplicationsELISA
ReactivityHuman
Sensitivity0.124ng/mL
Detection Limit0.312-20ng/mL
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Storage InstructionThe whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time3 hrs

Target Information

Gene SymbolPRKAB1
Gene ID5564
Uniprot IDAAKB1_HUMAN
Immunogen RegionReady-To-Use
Sample Typetissue homogenates or other biological fluids.

Additional Info

Tissue Specificity
Post Translational Modifications Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1.leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK.
Function Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton.probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
Protein Name 5'-Amp-Activated Protein Kinase Subunit Beta-1
Ampk Subunit Beta-1
Ampkb
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-1632852
Reactome: R-HSA-2151209
Reactome: R-HSA-380972
Reactome: R-HSA-5628897
Reactome: R-HSA-6804756
Reactome: R-HSA-9613354
Reactome: R-HSA-9619483
Cellular Localisation
Alternative ELISA Names 5'-Amp-Activated Protein Kinase Subunit Beta-1 ELISA kit
Ampk Subunit Beta-1 ELISA kit
Ampkb ELISA kit
PRKAB1 ELISA kit
AMPK ELISA kit
output

Information sourced from Uniprot.org

Citations

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