Human PR3 (Myeloblastin) Antibody Pair (STJAP00228)
General Information
| Short Description | This PR3 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Sheep/Sheep |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Polyclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | PRTN3 |
| Gene ID | 5657 |
| Uniprot ID | PRTN3_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human PR3 protein.Detection Immunogen: Recombinant Human PR3 protein. |
Additional Info
| Function | Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration. Plays a role in neutrophil transendothelial migration, probably when associated with CD177. Triggers inflammatory processes in neutrophils by interacting with ADGRG3 upstream of F2RL1/PAR2 activation. |
| Protein Name | MyeloblastinAgp7C-Anca AntigenLeukocyte Proteinase 3Pr-3Pr3Neutrophil Proteinase 4Np-4P29Wegener Autoantigen |
| Database Links | Reactome: R-HSA-140875Reactome: R-HSA-449836Reactome: R-HSA-6798695Reactome: R-HSA-6803157 |
| Cellular Localisation | Cytoplasmic GranuleSecretedCell MembranePeripheral Membrane ProteinExtracellular SideMembrane RaftLocalizes Predominantly To Azurophil Granules (Primary Secretory Granules) In NeutrophilsSecreted Upon Neutrophil Stimulation By Tnf-AlphaLipopolysaccharide (Lps)Fmlp And Cxcl8/Il8 Or During Neutrophil TransmigrationFollowing Secretion Tethered To The Cell Membrane By Cd177 |
| Alternative Antibody Pair Names | Myeloblastin antibody pairAgp7 antibody pairC-Anca Antigen antibody pairLeukocyte Proteinase 3 antibody pairPr-3 antibody pairPr3 antibody pairNeutrophil Proteinase 4 antibody pairNp-4 antibody pairP29 antibody pairWegener Autoantigen antibody pairPRTN3 antibody pairMBN antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human PR3 (Myeloblastin) Antibody Pair (STJAP00228)
This antibody pair detects human PR3 (Myeloblastin), corresponding to UniProt accession P24158. Reactivity is confirmed for human samples only.
The capture antibody is unconjugated and the detection antibody is biotinylated. Both are polyclonal antibodies raised in sheep, supplied at 0.5 mg/mL.
This antibody pair has been validated for use in ELISA. No other assay formats are listed for this product.
Both the capture and detection antibodies were raised against recombinant human PR3 protein. Both were subsequently purified by antigen affinity chromatography.
Both the capture and detection antibodies are formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4. Both components should be stored at -20°C for long-term storage, and freeze-thaw cycles should be avoided.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.