This PPIA Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications
ELISA
Reactivity
Human
Sensitivity
1.88ng/mL
Detection Limit
3.13~200ng/mL
Note
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Product Properties
Storage Instruction
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
This kit recognizes Human CYPA in samples. No significant cross-reactivity or interference between Human CYPA and analogues was observed.
Sample Type
Serum, plasma and other biological fluids
Additional Info
Function
Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Exerts a strong chemotactic effect on leukocytes partly through activation of one of its membrane receptors BSG/CD147, initiating a signaling cascade that culminates in MAPK/ERK activation. Activates endothelial cells (ECs) in a pro-inflammatory manner by stimulating activation of NF-kappa-B and ERK, JNK and p38 MAP-kinases and by inducing expression of adhesion molecules including SELE and VCAM1. Induces apoptosis in ECs by promoting the FOXO1-dependent expression of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis. In response to oxidative stress, initiates proapoptotic and antiapoptotic signaling in ECs via activation of NF-kappa-B and AKT1 and up-regulation of antiapoptotic protein BCL2. Negatively regulates MAP3K5/ASK1 kinase activity, autophosphorylation and oxidative stress-induced apoptosis mediated by MAP3K5/ASK1. Necessary for the assembly of TARDBP in heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and regulates TARDBP binding to RNA UG repeats and TARDBP-dependent expression of HDAC6, ATG7 and VCP which are involved in clearance of protein aggregates. Plays an important role in platelet activation and aggregation. Regulates calcium mobilization and integrin ITGA2B:ITGB3 bidirectional signaling via increased ROS production as well as by facilitating the interaction between integrin and the cell cytoskeleton. Binds heparan sulfate glycosaminoglycans. Inhibits replication of influenza A virus (IAV). Inhibits ITCH/AIP4-mediated ubiquitination of matrix protein 1 (M1) of IAV by impairing the interaction of ITCH/AIP4 with M1, followed by the suppression of the nuclear export of M1, and finally reduction of the replication of IAV. (Microbial infection) May act as a mediator between human SARS coronavirus nucleoprotein and BSG/CD147 in the process of invasion of host cells by the virus. (Microbial infection) Stimulates RNA-binding ability of HCV NS5A in a peptidyl-prolyl cis-trans isomerase activity-dependent manner.
Protein Name
Peptidyl-Prolyl Cis-Trans Isomerase APpiase ACyclophilin ACyclosporin A-Binding ProteinRotamase A Cleaved Into - Peptidyl-Prolyl Cis-Trans Isomerase A - N-Terminally Processed
CytoplasmSecretedNucleusSecretion Occurs In Response To Oxidative Stress In Vascular Smooth Muscle Through A Vesicular Secretory Pathway That Includes Rho Gtpase SignalingActin RemodelingAnd Myosin Ii Activation
Alternative ELISA Names
Peptidyl-Prolyl Cis-Trans Isomerase A ELISA kitPpiase A ELISA kitCyclophilin A ELISA kitCyclosporin A-Binding Protein ELISA kitRotamase A Cleaved Into - Peptidyl-Prolyl Cis-Trans Isomerase A - N-Terminally Processed ELISA kitPPIA ELISA kitCYPA ELISA kit
Specificity
This kit recognizes Human CYPA in samples. No significant cross-reactivity or interference between Human CYPA and analogues was observed.
