| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This PPARGC1A Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
PPARGC1A |
| Gene ID: |
10891 |
| Uniprot ID: |
PRGC1_HUMAN |
| Specificity: |
This kit recognizes Human PPAR Gamma C1 Alpha in samples. No significant cross-reactivity or interference between Human PPAR Gamma C1 Alpha and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2. Heavily acetylated by KAT2A/GCN5 under conditions of high nutrients, leading to inactivation of PPARGC1A. Deacetylated by SIRT1 in low nutrients/high NAD conditions, leading to its activation. Ubiquitinated. Ubiquitination by RNF34 induces proteasomal degradation. |
| Function | Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Acts as a key regulator of gluconeogenesis: stimulates hepatic gluconeogenesis by increasing the expression of gluconeogenic enzymes, and acting together with FOXO1 to promote the fasting gluconeogenic program. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK. |
| Protein Name | Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-AlphaPgc-1-AlphaPpar-Gamma Coactivator 1-AlphaPpargc-1-AlphaLigand Effect Modulator 6 |
| Database Links | Reactome: R-HSA-1989781Reactome: R-HSA-2151201Reactome: R-HSA-2151209Reactome: R-HSA-381340Reactome: R-HSA-3899300Reactome: R-HSA-400253Reactome: R-HSA-8939902Reactome: R-HSA-9615017Reactome: R-HSA-9707616 |
| Cellular Localisation | Isoform 1: NucleusNucleusPml BodyIsoform B4: NucleusIsoform B4-8a: CytoplasmIsoform B5: NucleusIsoform 9: Nucleus |
| Alternative ELISA Names | Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-Alpha ELISA kitPgc-1-Alpha ELISA kitPpar-Gamma Coactivator 1-Alpha ELISA kitPpargc-1-Alpha ELISA kitLigand Effect Modulator 6 ELISA kitPPARGC1A ELISA kitLEM6 ELISA kitPGC1 ELISA kitPGC1A ELISA kitPPARGC1 ELISA kit |
| Specificity | This kit recognizes Human PPARγC1α in samples. No significant cross-reactivity or interference between Human PPARγC1α and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-101 | 96 |
| EDTA plasma (n=8) | 94-107 | 101 |
| Cell culture media (n=8) | 95-109 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.51 | 1.32 | 4.51 | 0.47 | 1.43 | 4.38 |
| Standard deviation | 0.03 | 0.06 | 0.15 | 0.03 | 0.08 | 0.19 |
| CV (%) | 5.88 | 4.55 | 3.33 | 6.38 | 5.59 | 4.34 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
