Human PP2C alpha/PPM1A protein (Recombinant) (His-Tag) (STJP019729)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human PP2C alpha/PPM1A-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE/Enzyme Activity |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 10mM Tris-HCl buffer (pH 7.5) containing 50mM NaCl, 2mM DTT, 1mM MnCl2, 20%Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
| Immunoreactivity | Specific activity is > 1, 400unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C. |
Target Information
| Gene Symbol | PPM1A |
| Gene ID | 5494 |
| Uniprot ID | PPM1A_HUMAN |
| Accession Number | NP_066283 |
| Immunogen | Human |
| Immunogen Region | 1-382aa |
| Immunogen Sequence |
Additional Info
| Post Translational Modifications | N-myristoylation is essential for the recognition of its substrates for dephosphorylation. |
| Function | Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB. |
| Protein Name | Protein Phosphatase 1aProtein Phosphatase 2c Isoform AlphaPp2c-AlphaProtein Phosphatase Ia |
| Database Links | Reactome: R-HSA-2173795Reactome: R-HSA-380972 |
| Cellular Localisation | NucleusCytoplasmCytosolMembraneLipid-AnchorWeakly Associates At The Membrane And N-Myristoylation Mediates The Membrane Localization |
| Alternative Protein Names | Protein Phosphatase 1a proteinProtein Phosphatase 2c Isoform Alpha proteinPp2c-Alpha proteinProtein Phosphatase Ia proteinPPM1A proteinPPPM1A protein |
Information sourced from Uniprot.org