Human PON1 (Serum paraoxonase/arylesterase 1) Sandwich ELISA Kit (STJE0003597)

Name: Human PON1 (Serum paraoxonase/arylesterase 1) Sandwich ELISA Kit (STJE0003597)
SKU: STJE0003597
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0003597

Current Stock:

🚚 Free UK Delivery on orders over £150

Quantity:

Processing The item has been added

Enquire For Bulk Order

✓ 1-Year Performance Guarantee | SDS & Datasheet Included | Expert Tech Support | Covered by St John's Laboratory Guarantee

Short Description	This PON1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	0.09ng/mL
Detection Limit	0.16~10ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	PON1
Gene ID	5444
Uniprot ID	PON1_HUMAN
Specificity	This kit recognizes Human PON1 in samples. No significant cross-reactivity or interference between Human PON1 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Post Translational Modifications	Glycosylated. The signal sequence is not cleaved. Present in two forms, form B contains a disulfide bond, form A does not.
Function	Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.
Protein Name	Serum Paraoxonase/Arylesterase 1 Pon 1 Aromatic Esterase 1 A-Esterase 1 K-45 Serum Aryldialkylphosphatase 1
Database Links	Reactome: R-HSA-2142688 Reactome: R-HSA-9754706
Cellular Localisation	Secreted Extracellular Space
Alternative ELISA Names	Serum Paraoxonase/Arylesterase 1 ELISA kit Pon 1 ELISA kit Aromatic Esterase 1 ELISA kit A-Esterase 1 ELISA kit K-45 ELISA kit Serum Aryldialkylphosphatase 1 ELISA kit PON1 ELISA kit PON ELISA kit
Specificity	This kit recognizes Human PON1 in samples. No significant cross-reactivity or interference between Human PON1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.46	1.53	4.72	0.45	1.47	4.63
Standard deviation	0.03	0.06	0.26	0.03	0.06	0.19
CV (%)	6.52	3.92	5.51	6.67	4.08	4.10