| Host: | E.coli |
| Applications: | ELISA/WB |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Recombinant-Human POLB-C-His protein was developed from e.coli and has a target region of C-His. For use in research applications. |
| Conjugation: | Unconjugated |
| Formulation: | Supplied as a 0.22 Mu m filtered solution in 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 1mM EDTA, 50% Glycerol, pH 7.8. |
| Storage Instruction: | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin: | < 1 EU/µg as determined by LAL test. |
| Gene Symbol: | POLB |
| Gene ID: | 5423 |
| Uniprot ID: | DPOLB_HUMAN |
| Immunogen Region: | Ser2-Glu335 |
| Immunogen: | Homo sapiens (Human) |
| Function | Repair polymerase that plays a key role in base-excision repair. During this process, the damaged base is excised by specific DNA glycosylases, the DNA backbone is nicked at the abasic site by an apurinic/apyrimidic (AP) endonuclease, and POLB removes 5'-deoxyribose-phosphate from the preincised AP site acting as a 5'-deoxyribose-phosphate lyase (5'-dRP lyase).through its DNA polymerase activity, it adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. It is also able to cleave sugar-phosphate bonds 3' to an intact AP site, acting as an AP lyase. |
| Protein Name | Dna Polymerase Beta5'-Deoxyribose-Phosphate Lyase5'-Drp LyaseAp Lyase |
| Database Links | Reactome: R-HSA-110362Reactome: R-HSA-110373Reactome: R-HSA-110381Reactome: R-HSA-5649702Reactome: R-HSA-5651801Reactome: R-HSA-5689880Reactome: R-HSA-73930 |
| Cellular Localisation | NucleusCytoplasmCytoplasmic In Normal ConditionsTranslocates To The Nucleus Following Dna Damage |
| Alternative Protein Names | Dna Polymerase Beta protein5'-Deoxyribose-Phosphate Lyase protein5'-Drp Lyase proteinAp Lyase proteinPOLB protein |
Information sourced from Uniprot.org