Human POLA1 protein (Recombinant) (N-His) (STJP003916)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human POLA1-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | POLA1 |
| Gene ID | 5422 |
| Uniprot ID | DPOLA_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Gly1240-Ser1462 |
Additional Info
| Function | Catalytic subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, a regulatory subunit POLA2 and two primase subunits PRIM1 and PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. In the cytosol, responsible for a substantial proportion of the physiological concentration of cytosolic RNA:DNA hybrids, which are necessary to prevent spontaneous activation of type I interferon responses. |
| Protein Name | Dna Polymerase Alpha Catalytic SubunitDna Polymerase Alpha Catalytic Subunit P180 |
| Database Links | Reactome: R-HSA-113501Reactome: R-HSA-174411Reactome: R-HSA-174430Reactome: R-HSA-68952Reactome: R-HSA-68962Reactome: R-HSA-69091Reactome: R-HSA-69166Reactome: R-HSA-69183Reactome: R-HSA-69205Reactome: R-HSA-9710421 |
| Cellular Localisation | NucleusCytoplasmCytosolIn The CytosolColocalizes With Rna:dna Hybrids With A Speckled Pattern |
| Alternative Protein Names | Dna Polymerase Alpha Catalytic Subunit proteinDna Polymerase Alpha Catalytic Subunit P180 proteinPOLA1 proteinPOLA protein |
Information sourced from Uniprot.org