Human POFUT1 protein (Recombinant) (N-GST & C-His) (STJP011000)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human POFUT1-N-GST & C-His protein was developed from e.coli for the region N-GST & C-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | POFUT1 |
| Gene ID | 23509 |
| Uniprot ID | OFUT1_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Leu284-Asp379 |
Additional Info
| Post Translational Modifications | N-glycosylated. |
| Function | Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-S/T-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs). |
| Protein Name | Gdp-Fucose Protein O-Fucosyltransferase 1Peptide-O-Fucosyltransferase 1O-Fuct-1 |
| Database Links | Reactome: R-HSA-1912399 |
| Cellular Localisation | Endoplasmic Reticulum |
| Alternative Protein Names | Gdp-Fucose Protein O-Fucosyltransferase 1 proteinPeptide-O-Fucosyltransferase 1 proteinO-Fuct-1 proteinPOFUT1 proteinFUT12 proteinKIAA0180 protein |
Information sourced from Uniprot.org