Name: Human PLGF protein (Recombinant) (STJP000238)
SKU: STJP000238
Availability: InStock

General Information
Product Properties
Target Information
Additional Info
Product Review

Host:	CHO cells
Note:	STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description :	Recombinant-Human PLGF-protein was developed from cho cells. For use in research applications.

Conjugation:	Unconjugated
Formulation:	Lyophilised from 0.2 Mu m filtered solution in PBS pH 7.0.
Dilution Range:	Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers.
Storage Instruction:	Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA
Endotoxin:	Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA
Immunoreactivity:	The ED (50) was determined by the dose-dependent stimulation of theproliferation of human MDA cells is ≤ 10ug/ml. NA

Gene Symbol:	KITLG
Gene ID:	4254
Uniprot ID:	SCF_HUMAN
Immunogen Region:	Mature chain
Immunogen:	Optimized DNA sequence encoding HumanPlacenta growth factor isoform 1 mature chain fused to a C terminal Fc tag was expressed in CHO Cells NA

Post Translational Modifications	A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain. Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97. A soluble form exists as a cleavage product of the extracellular domain.
Function	Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
Protein Name	Kit Ligand Mast Cell Growth Factor Mgf Stem Cell Factor Scf C-Kit Ligand Cleaved Into - Soluble Kit Ligand Skitlg
Database Links	Reactome: R-HSA-1257604 P21583-1 Reactome: R-HSA-1433557 P21583-1 Reactome: R-HSA-1433559 P21583-1 Reactome: R-HSA-2219530 P21583-1 Reactome: R-HSA-5673001 P21583-1 Reactome: R-HSA-6811558 P21583-1 Reactome: R-HSA-9856649 P21583-1
Cellular Localisation	Isoform 1: Cell Membrane Single-Pass Type I Membrane Protein Isoform 2: Cytoplasm Cytoplasm Cytoskeleton Cell Membrane Cell Projection Lamellipodium Filopodium Soluble Kit Ligand: Secreted
Alternative Protein Names	Kit Ligand protein Mast Cell Growth Factor protein Mgf protein Stem Cell Factor protein Scf protein C-Kit Ligand Cleaved Into - Soluble Kit Ligand protein Skitlg protein KITLG protein MGF protein SCF protein

Information sourced from Uniprot.org

Model	Name	Host
STJP002466	Human Placenta growth factor/PLGF-131 protein (Recombinant) (C-hFc) (STJP002466)	HEK293 cells
STJP001156	Mouse Placenta growth factor/PlGF/PGF protein (Recombinant) (C-hFc&His) (STJP001156)	HEK293 cells
STJP000561	Human Phospholipid Transfer Protein protein (Recombinant) (STJP000561)	HEK293
STJP001872	Human Furin protein (Recombinant) (STJP001872)	HEK293
STJP001861	Human NKp44 protein (Recombinant) (STJP001861)	HEK293

Human PLGF protein (Recombinant) (STJP000238)

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