Human Platelet Derived Growth Factor-BB protein (Recombinant) (STJP000308)
SPECIFICATIONS
HostS.cerevisiae
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encoding Human PDGF-BB mature chain was expressed in Saccharomyces cerevisiae. NA
General Information
| Short Description | Recombinant-Human Platelet Derived Growth Factor-BB-protein was developed from s.cerevisiae. For use in research applications. |
| Host | S.cerevisiae |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Formulation | Lyophilised from 0.2 Mu m filtered.02M acetic acid solution pH.5. |
| Storage Instruction | Can be stored in working aliquots at°C-°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg (1EU/µg). NA |
| Immunoreactivity | The ED (50) was determined by the dose-dependent proliferation of Balb/cT3 cells.The expected ED50 for this effect is 1 ng/ml, corresponding to 1x10^6 IU/mg. NA |
Target Information
| Gene Symbol | PDGFB |
| Gene ID | 5155 |
| Uniprot ID | PDGFB_HUMAN |
| Immunogen | Optimized DNA sequence encoding Human PDGF-BB mature chain was expressed in Saccharomyces cerevisiae. NA |
| Immunogen Region | Mature chain |
| Immunogen Sequence | MNRCWALFLS LCCYLRLVSA EGDPIPEELY EMLSDHSIRS FDDLQRLLHG DPGEEDGAEL DLNMTRSHSG GELESLARGR RSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT RSPGGSQEQR AKTPQTRVTI RTVRVRRPPK GKHRKFKH |
Additional Info
| Tissue Specificity | Expressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung. |
| Function | Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA. |
| Protein Name | Platelet-Derived Growth Factor Subunit BPdgf Subunit BPdgf-2Platelet-Derived Growth Factor B ChainPlatelet-Derived Growth Factor Beta PolypeptideProto-Oncogene C-SisBecaplermin |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1257604Reactome: R-HSA-186763Reactome: R-HSA-186797Reactome: R-HSA-2219530Reactome: R-HSA-3000171Reactome: R-HSA-5673001Reactome: R-HSA-6811558 |
| Cellular Localisation | SecretedReleased By Platelets Upon Wounding |
| Alternative Protein Names | Platelet-Derived Growth Factor Subunit B proteinPdgf Subunit B proteinPdgf-2 proteinPlatelet-Derived Growth Factor B Chain proteinPlatelet-Derived Growth Factor Beta Polypeptide proteinProto-Oncogene C-Sis proteinBecaplermin proteinPDGFB proteinPDGF2 proteinSIS protein |
Information sourced from Uniprot.org