Human Phospholipase A2/PLA2G1B protein (Recombinant) (C-His) (STJP003076)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Phospholipase A2/PLA2G1B Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Ser148) of human Phospholipase A2/PLA2G1B (Accession #NP_000919.1) fused with His tag at the C-termi
General Information
| Short Description | Recombinant-Human Phospholipase A2/PLA2G1B-C-His protein was developed in hek293 cells using the region Met1-Ser148. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of 10 mM Tris, 5 mM CaCl2, pH 8.0. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its ability to hydrolyze 1-Hexadecanoyl-2- (1-pyrenedecanoyl)-sn-glycero-3-phosphomethanol. The specific activity is >2500 pmol/min/Mu g. |
| Determination Method | < 1.0 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | PLA2G1B |
| Gene ID | 5319 |
| Uniprot ID | PA21B_HUMAN |
| Immunogen | Recombinant Human Phospholipase A2/PLA2G1B Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Ser148) of human Phospholipase A2/PLA2G1B (Accession #NP_000919.1) fused with His tag at the C-termi |
| Immunogen Region | Met1-Ser148 |
| Immunogen Sequence | AVAEMNPNVNVFVPPRDGFS GPAPRKSKLICEATNFTPKP ITVSWLKDGKLVESGFTTDP VTIENKGSTPQTYKVISTLT ISEIDWLNLNVYTCRVDHRG LTFLKNVSSTCAASPSTDIL TFTIPPSFADIFLSKSANLT CLVSNLATYETLNISWASQS GEPLETKIKIMESHPNGTFS AKGVASVCVEDWNNRKEFVC TVTHRDLPSPQKKFISKPNE VHKHPPAVYLLPPAREQLN |
Additional Info
| Tissue Specificity | Selectively expressed in pancreas, lung, liver and kidney. Also detected at lower levels in ovary and testis. |
| Post Translational Modifications | Activated by trypsin cleavage in the duodenum. Can also be activated by thrombin or autocatalytically. |
| Function | Secretory calcium-dependent phospholipase A2 that primarily targets dietary phospholipids in the intestinal tract. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. May play a role in the biosynthesis of N-acyl ethanolamines that regulate energy metabolism and inflammation in the intestinal tract. Hydrolyzes N-acyl phosphatidylethanolamines to N-acyl lysophosphatidylethanolamines, which are further cleaved by a lysophospholipase D to release N-acyl ethanolamines. May act in an autocrine and paracrine manner. Upon binding to the PLA2R1 receptor can regulate podocyte survival and glomerular homeostasis. Has anti-helminth activity in a process regulated by gut microbiota. Upon helminth infection of intestinal epithelia, directly affects phosphatidylethanolamine contents in the membrane of helminth larvae, likely controlling an array of phospholipid-mediated cellular processes such as membrane fusion and cell division while providing for better immune recognition, ultimately reducing larvae integrity and infectivity. |
| Protein Name | Phospholipase A2Group Ib Phospholipase A2Phosphatidylcholine 2-Acylhydrolase 1b |
| Database Links | Reactome: R-HSA-1482788Reactome: R-HSA-1482801Reactome: R-HSA-1482839Reactome: R-HSA-1482922Reactome: R-HSA-1482925Reactome: R-HSA-1483166Reactome: R-HSA-9925561 |
| Cellular Localisation | SecretedSecreted From Pancreatic Acinar Cells In Its Inactive Form |
| Alternative Protein Names | Phospholipase A2 proteinGroup Ib Phospholipase A2 proteinPhosphatidylcholine 2-Acylhydrolase 1b proteinPLA2G1B proteinPLA2 proteinPLA2A proteinPPLA2 protein |
Information sourced from Uniprot.org